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| Application of loop-mediated isothermal amplification in detection of lower respiratory tract pathogens in patients with mechanical ventilation#br# |
| LIU Jiajia SUN Jingna YAN Haiyang HAN Zhengang CHEN Jing ZHAO Shuai▲ |
| Laboratory Center, the First Hospital of Hebei Medical University, Hebei Province, Shijiazhuang 050031, China |
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Abstract Objective To explore the application of loop-mediated isothermal amplification in the detection of lower respiratory tract pathogens in patients with mechanical ventilation. Methods From January to June 2021, a total of 90 patients with lower respiratory tract infection who were newly transferred to the Department of Respiratory Medicine and the Intensive Care Unit of the First Hospital of Hebei Medical University and underwent mechanical ventilation combined with ventilation through tracheal intubation were collected for bronchoalveolar lavage fluid samples for bacterial culture and drug sensitivity identification. At the same time, the loop-mediated isothermal amplification was used to detect the nucleic acid of pathogenic bacteria, and the samples of pathogenic bacteria were detected by drug resistance gene chip to analyze the detection of pathogens and drug resistance genes in patients. Results A total of 90 specimens were tested, among which 69 specimens were positive by the loop-mediated isothermal amplification, with a positive rate of 76.67%, and 31 were positive by bacterial culture, with a positive rate of 34.44%. The positive detection rate of the loop-mediated isothermal amplification was significantly higher than that of the bacterial culture method. The drug resistance test was carried out on the positive strains. Taking each sample as a separate whole, the loop-mediated isothermal amplification had a high detection rate in the detection of mecA, OXA-48, OXA-23, and other drug resistance genes, and was consistent with the detection rate of drug resistance genes. The compliance rate of the “gold standard” for drug resistance detection was high. Conclusion The detection rate of pathogens and drug resistance genes by the loop-mediated isothermal amplification is higher than that of traditional bacterial culture and drug susceptibility tests, and the detection rate of drug resistance genes is high, which has a good application prospect for guiding clinical medication.
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