|
|
|
| Exploration of the material basis and molecular mechanism of Rhizoma Polygonati in the treatment of non-small cell lung cancer based on network pharmacology and molecular docking#br# |
| YANG Jiani1 ZHU Xingmei1 YUAN Panpan2 GUO Kaili2 XUE Miao2 LIU Jiping1 WANG Chuan1 WANG Bin1 |
1.Key Laboratory of Pharmacodynamics and Material Basis of Chinese Medicine, Shaanxi Administration of Traditional Chinese Medicine, Shaanxi University of Chinese Medicine, Shaanxi Province, Xianyang 712046, China;
2.Shaanxi Key Laboratory of Traditional Medicine Foundation and New Drug Research, Shaanxi University of Chinese Medicine, Shaanxi Province, Xianyang 712046, China |
|
|
|
Abstract Objective To explore the material basis and molecular mechanism of Rhizoma Polygonati in the treatment of non-small cell lung cancer (NSCLC) based on network pharmacology and molecular docking. Methods The active components and effect targets of Rhizoma Polygonati were searched in traditional Chinese medicine systems pharmacology database and analysis platform, the targets of NSCLC were searched in Online Mendelian Inheritance in Man, Human Genome Annotation database, and Therapeutic Target database, the component-disease common targets were integrated. DAVID database was used for gene ontology and kyoto encyclopedia of genes and genomes pathway enrichment analysis, the network of “component-target-pathway” was constructed by Cytoscape 3.6.1 software, and the network was analyzed by network analyzer plug-in. AutoDock 4.2 was used for molecular docking, CCK8 method and flow cytometry were used for studying the anti-proliferation and apoptosis induced effects of flavone of Rhizoma Polygonati on A549 cells in vitro. Results 8 components in Rhizoma Polygonati exerted anti-NSCLC effects through 43 targets; these mainly included cancer, apoptosis, hypoxia inducible factor-1, tumor necrosis factor and other signaling pathways; 3’-methoxydaidzein, baicalein, liquiritigenin, and 5,4’-dihydroxyflavone played major roles corresponding to 7 targets; the IC50 of 3’-methox- ydaidzein, baicalein, and liquiritigenin acted on A549 cells for 48 h were 6.88×10, 7.99, 4.34×10 μg/ml respectively; high dose baicalein could induce apoptosis of A549 cells in a concentration-dependent manner. Conclusion Flavonoids in Rhizoma Polygonati could suppress proliferation and induce apoptosis to play the role of anti-NSCLC.
|
|
|
|
|
|
| Cite this article: |
|
YANG Jiani1 ZHU Xingmei1 YUAN Panpan2 GUO Kaili2 XUE Miao2 LIU Jiping1 WANG Chuan1 WANG Bin1. Exploration of the material basis and molecular mechanism of Rhizoma Polygonati in the treatment of non-small cell lung cancer based on network pharmacology and molecular docking#br#[J]. 中国医药导报, 2022, 19(8): 16-20.
|
|
|
|
| URL: |
| https://www.yiyaodaobao.com.cn/EN/ OR https://www.yiyaodaobao.com.cn/EN/Y2022/V19/I8/16 |
[1] Kimberly D，Miranda F，Theresa HK，et al. Cancer statistics for adolescents and young adults，2020 Cancer statistics [J]. CA Cancer J Clin，2020，70（6）：443-459.
[2] Min HY，Lee HY. Mechanisms of resistance to chemotherapy in non-small cell lung cancer [J]. Arch Pharm Res，2021， 44（2）：146-164.
[3] Naito T，Shiraishi H，Fujiwara Y. Brigatinib and lorlatinib：their effect on ALK inhibitors in NSCLC focusing on resistant mutations and central nervous system metastases [J]. Jpn J Clin Oncol，2021，51（1）：37-44.
[4] 李星.治疗恶性肿瘤方剂中补益药的配伍应用探讨[D].南京：南京中医药大学，2017.
[5] 潘磊.治疗肺癌方剂中风药的配伍规律研究[D].成都：成都中医药大学，2019.
[6] Yang J，Zhu X，Yuan P，et al. Efficacy of traditional Chinese Medicine combined with chemotherapy in patients with non-small cell lung cancer（NSCLC）：a meta-analysis of randomized clinical trials [J]. Support Care Cancer，2020， 28（8）：3571-3579.
[7] 杨丹，田国庆.中医药治疗肺癌的实验研究进展[J].中国中药杂志，2009，34（18）：2405-2409.
[8] 蒋文仪，包艺运，都广礼.网络中药药理学研究进展[J].中医药信息，2018，35（5）：122-126.
[9] Wu XM，Wu CF. Network pharmacology：a new approach to unveiling traditional chinese medicine [J]. Chin J Nat Med，2015，13（1）：1-2.
[10] Li J，Yan L，Luo J，et al. Baicalein suppresses growth of non-small cell lung carcinoma by targeting MAP4K3 [J]. Biomed Pharmacother，2021，133：110965-110977.
[11] Zhang Z，Nong L，Chen M，et al. Baicalein suppresses vasculogenic mimicry through inhibiting RhoA/ROCK expression in lung cancer A549 cell line [J]. Acta Biochim Biophys Sin，2020，52（9）：1007-1015.
[12] 屈中玉，王文廉，王启船，等.汉黄芩素对肺癌肿瘤细胞生长和转移抑制作用机制的研究[J].中药药理与临床，2017，33（1）：81-85.
[13] 廉政君，常炜.汉黄芩素调节JAK/STAT信号通路并诱导肺癌A549细胞凋亡及周期阻滞的作用及机制研究[J].临床肺科杂志，2021，26（2）：270-274，279.
[14] 白吉庆.陕西道地药材——黄精[J].现代中医药，2019， 39（3）：136.
[15] 龙婷婷.基于TLR4-MAPK-NF-kB信号通路探讨黄精多糖免疫调节抗肿瘤作用机制研究[D].重庆：重庆医科大学，2018.
[16] 段华，王保奇，张跃文.黄精多糖对肝癌H-（22）移植瘤小鼠的抑瘤作用及机制研究[J].中药新药与临床药理，2014，25（1）：5-7.
[17] 张娇，王元忠，杨维泽，等.黄精属植物化学成分及药理活性研究进展[J].中国中药杂志，2019，44（10）：1989-2008.
[18] 杨楠，贾晓斌，张振海，等.黄酮类化合物抗肿瘤活性及机制研究进展[J].中国中药杂志，2015，40（3）：373-381.
[19] Chen JJ，Huang CC，Chang HY，et al. Scutellaria baicalensis Ameliorates Acute Lung Injury by Suppressing Inflammation In Vitro and In Vivo [J]. Am J Chin Med，2017，45（1）：137-157.
[20] 张晓龙.清热解毒法调控ezrin-SNO依赖的胞内张力抗NSCLC侵袭、转移的机制研究[D].南京：南京中医药大学，2019.
[21] Sammons MA，Nguyen TT，McDade SS，et al. Tumor suppressor p53：from engaging DNA to target gene regulation [J]. Nucleic Acids Res，2020，48（16）：8848-8869.
[22] Tian H，Lian R，Li Y，et al. AKT-induced lncRNA VAL promotes EMT-independent metastasis through diminishing Trim16-dependent Vimentin degradation [J]. Nat Commun，2020，11（1）：5127-5141.
[23] Song M，Bode AM，Dong Z,，et al. AKT as a Therapeutic Target for Cancer [J]. Cancer Res，2019，79（6）：1019-1031.
[24] Mayer IA，Arteaga CL. The PI3K/AKT Pathway as a Target for Cancer Treatment [J]. Annu Rev Med，2016，67（1）：11-28.
[25] Ladokhin AS. Regulation of Apoptosis by the Bcl-2 Family of Proteins：Field on a Brink [J]. Cells，2020，9（9）：2121-2122.
[26] 张佳秀，邓林锋，石建邦，等.非小细胞肺癌患者肿瘤微环境中TAMs及PD-1表达水平及与临床预后的关系[J].中国当代医药，2021，28（32）：4-8，241.
[27] Jiang M，Qi L，Li L，et al. The caspase-3/GSDME signal pathway as a switch between apoptosis and pyroptosis in cancer [J]. Cell Death Discov，2020，28（6）：112-122. |
|
|
|
