溃疡性结肠炎中巨噬细胞差异基因及功能分析

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Abstract Objective To study the difference in gene expression and biological functions of intestinal macrophages in ulcerative colitis (UC). Methods Seven UC patients and seven healthy controls who visited the VA San Diego Healthcare facility from January to December 2019 were selected as the study subjects. The colonic mucosal single cell sequencing data were analyzed, and the differentially expressed genes in macrophages were compared, then gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) enrichment analysis were performed. Results The cells were filtered and the cluster of macrophage was determined. A total of 31 differentially expressed genes were selected in intestinal mucosa of UC group and control group, including TIMP1, IFITM3, IGHG1, etc. GO and KEGG enrichment analysis of differentially expressed genes showed mainly enriched in the biological process of oxidative stress and IL-17 signaling pathway. Conclusion The oxidative stress and IL-17 signaling pathway of intestinal macrophages may be involved in the process of UC disease.

Key words： Ulcerative colitis Single cell sequencing Macrophages Oxidative stress Interleukin-17

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	CHEN Mengxue WANG Honggang XIE Rui▲

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CHEN Mengxue WANG Honggang XIE Rui▲. Analysis of differential genes and functions of macrophages in ulcerative colitis #br#[J]. 中国医药导报, 2022, 19(7): 27-30.

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https://www.yiyaodaobao.com.cn/EN/ OR https://www.yiyaodaobao.com.cn/EN/Y2022/V19/I7/27

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