|
|
|
| Effect of osteopontin on proliferation, invasion and migration of gastric cancer cells#br# |
| QIN Yuechao1 YUAN Xiaolin2 QU Fanjie1 |
1.Department of Oncology, Dalian Third People’s Hospital, Liaoning Province, Dalian 116033, China;
2.Central Laboratory, Affiliated Zhongshan Hospital of Dalian University, Liaoning Province, Dalian 116001, China |
|
|
|
Abstract Objective To investigate the effect of osteopontin (OPN) on the proliferation, invasion, and migration ability of gastric cancer SGC-7901 cells. Methods Gastric cancer SGC-7901 cells were cultured in vitro and they were divided into three groups: control group, NC-shRNA group, OPN-shRNA group. The proliferation of cell was detected by MTT assay, and the invasion and migration were detected by Transwell assay. The expression of cyclin D1, vascular endothelial growth factor (VEGF), and matrix metallo proteinase 2 (MMP-2) was detected by Western blot assay. Results There was no significant difference between control group and NC-shRNA group in cell proliferation, invasion, migration, and protein expression levels (P > 0.05). After silencing OPN gene expression, OPN-shRNA had significant cell proliferation, invasion, and migration capabilities were lower than control group, the differences were highly statistically significant (P < 0.01); the expression levels of OPN-shRNA cyclin D1, VEGF, and MMP-2 protein were lower than control group, and the difference was statistically significant (P < 0.05). Conclusion OPN can enhance the proliferation, invasion, and migration of gastric cancer SGC-7901 cells, and its mechanism may be related to the regulation of cyclin D1, VEGF, and MMP-2 expression.
|
|
|
|
|
|
[1] Bray F，Ferlay J，Soerjomataram I，et al. Global cancer statistics 2018：GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries [J]. CA Cancer J Clin，2018，68（6）：394-424.
[2] Zhang Z，Pi J，Zou D，et al. microRNA arm-imbalance in part from complementary targets mediated decay promotes gastric cancer progression [J]. Nat Commun，2019，10（1）：4397.
[3] Tse J，Pierce T，Carli ALE，et al. Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells [J]. Proteomics，2021，21（13/14）：e2000098.
[4] Turgeon MK，Lee RM，Keilson JM，et al. Is there a difference in utilization of a perioperative treatment approach for gastric cancer between safety net hospitals and tertiary referral centers? [J]. J Surg Oncol，2021，124（4）：551-559.
[5] Denhardt DT，Guo X. Osteopontin：a protein with diverse functions [J]. FASEB J，1993，7（15）：1475-1482.
[6] Xing X，Wang J，Xing LX，et al. Involvement of MAPK and PI3K signaling pathway in sterigmatocystin-induced G2 phase arrest in human gastric epithelium cells [J]. Mol Nutr Food Res，2011，55（5）：749-760.
[7] Rizwan A，Paidi SK，Zheng C，et al. Mapping the genetic basis of breast microcalcifications and their role in metastasis [J]. Sci Rep，2018，8（1）：11067.
[8] Amilca-Seba K，Sabbah M，Larsen AK，et al. Osteopontin as a Regulator of Colorectal Cancer Progression and Its Clinical Applications [J]. Cancers，2021，13（15）：3793.
[9] Dos Santos ES，Ramos JC，Roza ALOC，et al. The role of osteopontin in oral cancer： A brief review with emphasis on clinical applications [J]. Oral Dis，2020. Online ahead of print.
[10] Jing CY，Fu YP，Zhou C，et al. Hepatic stellate cells promote intrahepatic cholangiocarcinoma progression via NR4A2/osteopontin/Wnt signaling axis [J]. Oncogene，2021，40（16）：2910-2922.
[11] Subraman V，Thiyagarajan M，Malathi N，et al. OPN -Revisited [J]. J Clin Diagn Res，2015，9（6）：ZE10-3.
[12] Xu ST，Guo C，Ding X，et al. Role of osteopontin in the regulation of human bladder cancer proliferation and migration in T24 cells [J]. Mol Med Rep，2015，11（5）：3701-3707.
[13] Huang RH，Quan YJ，Chen JH，et al. Osteopontin Promotes Cell Migration and Invasion， and Inhibits Apoptosis and Autophagy in Colorectal Cancer by activating the p38 MAPK Signaling Pathway [J]. Cell Physiol Biochem，2017，41（5）：1851-1864.
[14] Lin CN，Wang CJ，Chao YJ，et al. The significance of the co-existence of osteopontin and tumor-associated mac-rophages in gastric cancer progression [J]. BMC Cancer，2015，15（15）：128.
[15] Kiss T，Jámbor K，Koroknai V，et al. Silencing Osteopontin Expression Inhibits Proliferation， Invasion and Induce Altered Protein Expression in Melanoma Cells [J]. Pathol Oncol Res，2021，5（27）：581395.
[16] Zhang X，Tsukamoto T，Mizoshita T，et al. Expression of osteopontin and CDX2：indications of phenotypes and prognosis in advanced gastric cancer [J]. Oncol Rep，2009， 21（3）：609-613.
[17] Hu H，Liu Z，Liu C. Correlation of OPN gene expression with proliferation and apoptosis of ovarian cancer cells and prognosis of patients [J]. Oncol Lett，2019，17（3）：2788-2794.
[18] Wang ZM，Cui YH，Li W，et al. Lentiviral-mediated siRNA targeted against osteopontin suppresses the growth and metastasis of gastric cancer cells [J]. Oncol Rep，2011， 25（4）：997-1003.
[19] Wang TC，Cardiff RD，Zukerberg L，et al. Mammary hyperplasia and carcinoma in MMTV-cyclin D1 transgenic mice [J]. Nature，1994，369（6482）：669-671.
[20] Jab?覥ońska-Trypu■ A，Matejczyk M，Rosochacki S. Matrix metalloproteinases（MMPs），the main extracellular matrix （ECM）enzymes in collagen degradation，as a target for anticancer drugs [J]. J Enzyme Inhib Med Chem，2016， 31（sup1）：177-183.
[21] Philip S，Bulbule A，Kundu GC. Osteopontin stimulates tumor growth and activation of promatrix metalloproteinase-2 through nuclear factor-kappa B-mediated induction of membrane type 1 matrix metalloproteinase in murine melanoma cells [J]. J Biol Chem，2001，276（48）：44926-44935.
[22] Lin Q，Guo L，Lin G，et al. Clinical and prognostic significance of OPN and VEGF expression in patients with non-small-cell lung cancer [J]. Cancer Epidemiol，2015， 39（4）：539-544.
[23] Xu ST，Zou FZ，Cai LN，et al. The downregulation of OPN inhibits proliferation and migration and regulate activation of Erk1/2 in ECA-109 cells [J]. Int J Clin Exp Med，2015，8（4）：5361-5369.
[24] Yan W，Qian C，Zhao P，et al. Expression pattern of osteopontin splice variants and its functions on cell apoptosis and invasion in glioma cells [J]. Neuro Oncol，2010， 12（8）：765-775.
[25] 顾宇，陆枫林.VEGF促进肝癌SMMC-7721细胞侵袭性的自分泌机制[J].世界华人消化杂志，2009，17（32）：3292-3295.
[26] 张金玲，王玥，张丽娟，等.VEGF促进人黑素瘤SK-MEL-1细胞侵袭性的自分泌机制初探[J].中国皮肤性病学杂志，2012，26（3）：192-195. |
|
|
|
