高糖环境下糖络宁对大鼠背根神经节神经元细胞凋亡水平及MAPKs信号通路的影响

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Abstract Objective To study the effect of Tangluo Ning on apoptosis and mitogen-activated protein kinases (MAPKs) signaling pathway of rat dorsal root ganglion neurons (DRGn) in high glucose environment. Methods Sixty clean grade male SD rats with body weight of (215±15) g at 6-8 weeks were divided into high, medium, and low concentration glucolonin groups and normal group according to random number table method, with 15 rats in each group. Each group was given 5, 2.5, 1.25 g/(kg·d) crude medicine of Tangluo Ning prescription and equal amount of distilled water intragastric administration to prepare drug-containing serum and normal control serum. DRGn cells were prepared from newborn SD fetal rats and were divided into normal group (10% normal rat serum +Neurobasal medium), high glucose group (based on normal group +50 mmol/L glucose), and Tangluo Ning concentration groups (based on high glucose group +10% low, medium, and high concentration Tangluo Ning drug-containing serum). Cell apoptosis and protein expression of JNK1, p-JNK1, c-Jun, p-c-Jun, Map3k8, and p-Map3k8 were detected after 24 h culture. Results The apoptosis rate, expression of p-JNK1, p-c-Jun, Map3k8, and p-Map3k8 and ratio of p-JNK1/JNK1, p-c-Jun/c-Jun, and p-Map3k8/Map3k8 in high glucose group were higher than those in normal group, the differences were statistically significant (P ＜ 0.05 or P ＜ 0.01). The apoptosis rate, expression of p-JNK1, p-c-Jun, and p-Map3k8 and ratio of p-JNK1/JNK1, p-c-Jun/c-Jun, and p-Map3k8/Map3k8 in Tangluo Ning concentration groups were significantly lower than those in high glucose group, the differences were highly statistically significant (P ＜ 0.01). Apoptosis rate, expression of p-JNK1, p-c-Jun, and p-Map3k8 and ratio of p-JNK1/JNK1, p-c-Jun/c-Jun, and p-Map3k8/Map3k8 in middle concentration Tangluo Ning group were significantly lower than those in high and low concentration Tangluo Ning group, the differences were statistically significant (P ＜ 0.01 or P ＜ 0.05). The expression of c-Jun in the medium and low concentration Tangluo Ning groups was lower than that in the high concentration Tangluo Ning group, and the differences were statistically significant (P ＜ 0.05). There was no significant difference in the expression of JNK1, Map3k8 and c-Jun in the Tangluo Ning concentration groups (P ＞ 0.05). Conclusion Tangluo Ning can reduce apoptosis by inhibiting MAPKs signaling pathway.

Key words： Dorsal root ganglion neurons Diabetic peripheral neuropathy Tangluo Ning Apoptosis

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	ZHU Yufei1 GUO Yuxin1 WANG Liying2 LI Jin1 ZHANG Taojing2

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ZHU Yufei1 GUO Yuxin1 WANG Liying2 LI Jin1 ZHANG Taojing2. Effects of Tangluo Ning on apoptosis and MAPKs signaling pathway of rat dorsal root ganglion neurons in high glucose environment#br#[J]. 中国医药导报, 2022, 19(4): 9-13.

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https://www.yiyaodaobao.com.cn/EN/ OR https://www.yiyaodaobao.com.cn/EN/Y2022/V19/I4/9

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