Role of endoplasmic reticulum stress pathway in palmitic acid-induced apoptosis of human periodontal membrane stem cells#br#
LIU Tao1,2 LI Jing2 BAO Cuiyu2
1.Department of Stomatology, Affiliated Second Hospital of Hubei University of Science and Technology, Hubei Province, Xianning 437100, China;
2.Hubei Province Key Laboratory on Cardiovascular, Cerebrovascular, and Metabolic Disorders, Hubei University of Science and Technology, Hubei Province, Xianning 437100, China
Abstract Objective To investigate the mechanism of endoplasmic reticulum stress pathway in the apoptosis of human periodontal ligament stem cells (hPDLSCs) induced by palmitic acid (PA). Methods The cultured hPDLSCs were randomly divided into control group (medium without PA), low, medium, and high concentrations of PA (0.1, 0.2, and 0.4 mmol/L) groups to explore the mechanism of PA inhibiting hPDLSCs proliferation; the cultured hPDLSCs were divided into control group (medium without PA), PA group (0.4 mmol/L) and PA (0.4 mmol/L) +4-PBA group (5 μmol/L) to explore the effect of 4-PBA on the apoptosis of hPDLSCs induced by PA. After 24 h of culture, the proliferation rate of hPDLSCs in each group was detected by MTT assay, the apoptosis of hPDLSCs in each group was observed by Annexin V-FITC/PI probe, and the expression of endoplasmic reticulum stress apoptosic-related proteins was detected by Western blot. Results The proliferation rate of hPDLSCs in low, medium, and high PA groups was significantly lower than that in control group (P ＜ 0.05). The nuclear red fluorescence and cell membrane green fluorescence of PA high concentration group were increased significantly compared with cortrol group. The expressions of IRE1phos, PERKphos, ATF4, ATF6, and CHOP in PA high concentration group were significantly higher than those in control group (P ＜ 0.05). Cleaved caspase-3 expression in PA medium and high concentration groups was significantly higher than that in control group (P ＜ 0.05). Compared with PA low and medium concentrations groups, IRE1phos, PERKphos, ATF6, ATF4, CHOP, and Cleaved caspase-3 protein expressions in PA high concentration group were significantly increased (P < 0.05). Compared with PA group, PERKphos, CHOP, and Cleaved caspase-3 expressions were significantly down-regulated in PA+4-PBA group (P ＜ 0.05). Conclusion PA may promote the apoptosis of hPDLSCs by regulating the protein expression of endoplasmic reticulum stress apoptosis pathway.
LIU Tao1,2 LI Jing2 BAO Cuiyu2. Role of endoplasmic reticulum stress pathway in palmitic acid-induced apoptosis of human periodontal membrane stem cells#br#[J]. 中国医药导报, 2022, 19(4): 4-8.
