Abstract of Paediatrics, Longquanyi District of Chengdu Maternity and Child Health Care Hospital, Sichuan Province, Chengdu 610100, China; 2.Department of Obstetrics and Gynecology, Longquanyi District of Chengdu Maternity and Child Health Care Hospital, Sichuan Province, Chengdu 610100, China; 3.Division of Neonatology, Longquanyi District of Chengdu Maternity and Child Health Care Hospital, Sichuan Province, Chengdu 610100, China
[Abstract] Objective To investigate the relationship between microRNA (miRNA)-138 expression and T helper cell (Th)1/Th2 balance in peripheral blood of children with bronchial asthma (BA). Methods A total of 103 children with BA admitted to Longquanyi District of Chengdu Maternity and Child Health Care Hospital from January 2020 to March 2021 were selected. According to clinical manifestations, they were divided into acute attack group (42 cases) and clinical remission group (61 cases), and 82 healthy children with physical examination were selected as control group during the same period. Peripheral blood miR-138, T-bet, GATA binding protein 3 (GATA3) detected by qRT-PCR, peripheral blood Th1 percentage and Th2 percentage detected by flow cytometry, and peripheral blood interleukin-2 (IL-2), IL-4, IL-10, and interferon-γ (INF-γ) levels were detected by enzyme linked immunosorbent assay. Results MiR-138 expression and Th2 percentage in peripheral blood of acute attack group were higher than those of clinical remission group and control group, while Th1 percentage and Th1/Th2 were lower than those of clinical remission group and control group (P < 0.05); MiR-138 expression and Th2 percentage in peripheral blood in clinical remission group were higher than those in control group, while Th1 percentage and Th1/Th2 were lower than those in control group (P < 0.05). T-bet level in peripheral blood of acute attack group was lower than that of clinical remission group and control group, while GATA3 level was higher than that of clinical remission group and control group (P < 0.05); T-bet level in peripheral blood of clinical remission group was lower than that of control group, and GATA3 was higher than that of control group (P < 0.05). The levels of IL-2 and IFN-γ in peripheral blood of acute attack group were lower than those of clinical remission group and control group, while the levels of IL-4 and IL-10 were higher than those of clinical remission group and control group (P < 0.05); the levels of IL-2 and IFN-γ in peripheral blood in clinical remission group were lower than those in control group, while the levels of IL-4 and IL-10 were higher than those in control group (P < 0.05). There were negative correlation between miR-138 expression and T-bet, Th1/Th2, IL-2, and IFN-γ in peripheral blood of children with BA (r = -0.498, -0.515, -0.464, -0.428, P < 0.01); it was positively correlated with GATA3, IL-4, and IL-10 (r = 0.595, 0.518, 0.386, P < 0.01). Conclusion Peripheral blood miR-138 expression is significantly upregulated in children with BA, which may mediate the inflammatory response by regulating the Th1/Th2 balance, participate in the development of BA occurs.
LIAO Quanhui1 WANG Shuirong1 HUANG Long1 CAI Xue1 FU Tianming2 LI Kexing3. Relationship between miR-138 expression and Th1/Th2 balance in peripheral blood of children with bronchial asthma#br#[J]. 中国医药导报, 2022, 19(3): 87-90,98.
[1] 中华医学会呼吸病学分会哮喘学组.支气管哮喘防治指南（2020年版）[J].中华结核和呼吸杂志，2020，43（12）：1023-1048.
[2] 中华儿科杂志编辑委员会，中华医学会儿科学分会呼吸学组，中国医师协会儿科医师分会儿童呼吸专业委员会.儿童支气管哮喘规范化诊治建议（2020年版）[J].中华儿科杂志，2020，58（9）：708-717.
[3] Huang K，Yang T，Xu J，et al. Prevalence，risk factors，and management of asthma in China：a national cross-sectional study [J]. Lancet，2019，394（10196）：407-418.
[4] 黄伟强，李惠，袁梅，等.支气管哮喘患者外周血T细胞亚群 Th1、Th2及其相关细胞因子水平变化及意义[J].山东医药，2015（20）：40-41.
[5] Chiba Y. Non-coding RNAs and bronchial smooth muscle hyperresponsiveness in allergic bronchial asthma [J]. Nihon Yakurigaku Zasshi，2020，155（6）：364-368.
[6] Specjalski K，Jassem E. MicroRNAs：Potential Biomarkers and Targets of Therapy in Allergic Diseases? [J]. Arch Immunol Ther Exp（Warsz），2019，67（4）：213-223.
[7] Ye S，Zhu S，Feng L. LncRNA ANRIL/miR-125a axis exhibits potential as a biomarker for disease exacerbation，severity，and inflammation in bronchial asthma [J]. J Clin Lab Anal，2020，34（3）：e23092.
[8] 葛珊慧，曾勉.miR-142-3p在免疫炎症中研究进展[J].国际呼吸杂志，2020，40（10）：784-789.
[9] Walther K，Schulte LN. The role of lncRNAs in innate immunity and inflammation [J]. RNA Biol，2021，18（5）：587-603.
[10] 刘燕，李淑杰，罗云.过敏性哮喘患者外周血单个核细胞miR-138表达及与Th1/Th2平衡的关系[J].河北医药，2020，42（20）：3055-3059.
[11] Chunlei H，Chang Z，Sheng L，et al. Down-regulation of MiR-138-5p Protects Chondrocytes ATDC5 and CHON-001 from IL-1 β-induced Inflammation Via Up-regulating SOX9 [J]. Curr Pharm Des，2020，25（43）：4613-4621.
[12] 中华医学会儿科学分会呼吸学组，《中华儿科杂志》编辑委员会.儿童支气管哮喘诊断与防治指南（2016年版）[J].中华儿科杂志，2016，54（3）：167-181.
[13] 中华医学会，中华医学会杂志社，中华医学会全科医学分会，等.支气管哮喘基层诊疗指南（2018年）[J].中华全科医师杂志，2018，17（10）：751-762.
[14] Qiu L，Zhang Y，Do DC，et al. miR-155 Modulates Cockroach Allergen- and Oxidative Stress-Induced Cyclooxygenase-2 in Asthma [J]. J Immunol，2018，201（3）：916-929.
[15] Blanca A，Sanchez-Gonzalez A，Requena MJ，et al. Expression of miR-100 and miR-138 as prognostic biomarkers in non-muscle-invasive bladder cancer [J]. APMIS，2019，127（8）：545-553.
[16] 袁红刚，李三梅.MiR-138在脑胶质瘤患者血清表达的生物学效应及临床意义[J].转化医学杂志，2020，9（1）：4-7.
[17] Wei J，Nduom EK，Kong LY，et al. MiR-138 exerts anti-glioma efficacy by targeting immune checkpoints [J]. Neuro Oncol，2016，18（5）：639-648.
[18] Shao L，Hou C. miR-138 activates NF-κB signaling and PGRN to promote rheumatoid arthritis via regulating HDAC4 [J]. Biochem Biophys Res Commun，2019，519（1）：166-171.
[19] 薛玲娜，张惠勇，郭晓燕，等.固有淋巴细胞在哮喘发病机制及治疗中的作用[J].中华临床免疫和变态反应杂志，2019，13（5）：418-423.
[20] Kuo CS，Pavlidis S，Loza M，et al. T-helper cell type 2（Th2）and non-Th2 molecular phenotypes of asthma using sputum transcriptomics in U-BIOPRED [J]. Eur Respir J，2017，49（2）：1602135.
[21] Liang P，Peng S，Zhang M，et al. Huai Qi Huang corrects the balance of Th1/Th2 and Treg/Th17 in an ovalbumin-induced asthma mouse model [J]. Biosci Rep，2017，37（6）：BSR20171071.
[22] Matucci A，Vultaggio A，Maggi E，et al. Is IgE or eosinophils the key player in allergic asthma pathogenesis? Are we asking the right question? [J]. Respir Res，2018，19（1）：113.
[23] 邓玲，陈永平，孙燕玲.强直性脊柱炎患者外周血单核细胞miR-138表达及与Th1/Th2失衡的关系[J].热带医学杂志，2019，19（8）：1008-1011，1051.
[24] Ogino H，Fukaura K，Iboshi Y，et al. Role of the IL-23-T-bet/GATA3 Axis for the Pathogenesis of Ulcerative Colitis [J]. Inflammation，2021，44（2）：592-603.
[25] Li Y，Du X，Zhao Y，et al. Fluoride Can Damage the Spleen of Mice by Perturbing Th1/Th2 Cell Balance [J]. Biol Trace Elem Res，2021，199（4）：1493-1500.
