支气管哮喘患儿外周血中miR-138表达与Th1/Th2平衡的关系

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Abstract of Paediatrics, Longquanyi District of Chengdu Maternity and Child Health Care Hospital, Sichuan Province, Chengdu 610100, China; 2.Department of Obstetrics and Gynecology, Longquanyi District of Chengdu Maternity and Child Health Care Hospital, Sichuan Province, Chengdu 610100, China; 3.Division of Neonatology, Longquanyi District of Chengdu Maternity and Child Health Care Hospital, Sichuan Province, Chengdu 610100, China
[Abstract] Objective To investigate the relationship between microRNA (miRNA)-138 expression and T helper cell (Th)1/Th2 balance in peripheral blood of children with bronchial asthma (BA). Methods A total of 103 children with BA admitted to Longquanyi District of Chengdu Maternity and Child Health Care Hospital from January 2020 to March 2021 were selected. According to clinical manifestations, they were divided into acute attack group (42 cases) and clinical remission group (61 cases), and 82 healthy children with physical examination were selected as control group during the same period. Peripheral blood miR-138, T-bet, GATA binding protein 3 (GATA3) detected by qRT-PCR, peripheral blood Th1 percentage and Th2 percentage detected by flow cytometry, and peripheral blood interleukin-2 (IL-2), IL-4, IL-10, and interferon-γ (INF-γ) levels were detected by enzyme linked immunosorbent assay. Results MiR-138 expression and Th2 percentage in peripheral blood of acute attack group were higher than those of clinical remission group and control group, while Th1 percentage and Th1/Th2 were lower than those of clinical remission group and control group (P < 0.05); MiR-138 expression and Th2 percentage in peripheral blood in clinical remission group were higher than those in control group, while Th1 percentage and Th1/Th2 were lower than those in control group (P < 0.05). T-bet level in peripheral blood of acute attack group was lower than that of clinical remission group and control group, while GATA3 level was higher than that of clinical remission group and control group (P < 0.05); T-bet level in peripheral blood of clinical remission group was lower than that of control group, and GATA3 was higher than that of control group (P < 0.05). The levels of IL-2 and IFN-γ in peripheral blood of acute attack group were lower than those of clinical remission group and control group, while the levels of IL-4 and IL-10 were higher than those of clinical remission group and control group (P < 0.05); the levels of IL-2 and IFN-γ in peripheral blood in clinical remission group were lower than those in control group, while the levels of IL-4 and IL-10 were higher than those in control group (P < 0.05). There were negative correlation between miR-138 expression and T-bet, Th1/Th2, IL-2, and IFN-γ in peripheral blood of children with BA (r = -0.498, -0.515, -0.464, -0.428, P < 0.01); it was positively correlated with GATA3, IL-4, and IL-10 (r = 0.595, 0.518, 0.386, P < 0.01). Conclusion Peripheral blood miR-138 expression is significantly upregulated in children with BA, which may mediate the inflammatory response by regulating the Th1/Th2 balance, participate in the development of BA occurs.

Key words： Bronchial asthma MicroRNA-138 Helper T cell Inflammation

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	LIAO Quanhui1 WANG Shuirong1 HUANG Long1 CAI Xue1 FU Tianming2 LI Kexing3

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LIAO Quanhui1 WANG Shuirong1 HUANG Long1 CAI Xue1 FU Tianming2 LI Kexing3. Relationship between miR-138 expression and Th1/Th2 balance in peripheral blood of children with bronchial asthma#br#[J]. 中国医药导报, 2022, 19(3): 87-90,98.

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https://www.yiyaodaobao.com.cn/EN/ OR https://www.yiyaodaobao.com.cn/EN/Y2022/V19/I3/87

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