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| Effects of different frequencies electro-acupuncture on cerebral ischemia reperfusion model rats#br# |
| LIAO Heng1 FAN Rongguo2 LI Youchun2 GAO Yujiao1 MU Jingping1 |
1.Sleep Psychosomatic Medicine Center, Taihe Hospital of Shiyan City Affiliated Hospital of Hubei University of Medicine, Hubei Province, Shiyan 442000, China;
2.Department of Health Management, Danjiangkou First Hospital, Hubei Province, Danjiangkou 442700, China |
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Abstract Objective To observe the effects of different frequencies electro-acupuncture on cerebral ischemia reperfusion model rats. Methods A total of 50 SPF male SD rats with the body weight of (235±5) g were selected, and ten of them were randomly selected as the sham operation group. The rest were established by middle cerebral artery occlusion to establish cerebral ischemia reperfusion model. After the molding was successful, they were divided into model group and high, medium, low frequency electro-acupuncture groups by random number table method, with ten rats in each group. High (100 Hz), medium (50 Hz), and low (2 Hz) frequency electro-acupuncture groups were treated with electro-acupuncture at bǎihuì, shéntíng, and shuǐgōu, a total of four weeks of intervention, sham operation group and model group did not intervene. After four weeks, regional cerebral blood flow (rCBF), hemorheological indexes, osmotic fragility of erythrocyte (EOF), blood-brain barrier permeability (Evans blue content in brain tissue), sugar water preference test, and Morris water maze test were compared among five groups. Results In the model group, the whole blood viscosity (high, medium, low cut), plasma viscosity, red blood cell aggregation index, Evans blue content of brain tissue were all higher than those in sham operation group, the sugar water preference rate, EOF (maximum and minimum) were lower than those in the sham operation group, and the escape latency was longer than that in the sham operation group, and the number of table crossing was lower than that in the sham operation group, and the differences were statistically significant (P ＜ 0.05). In high, medium, low frequency electro-acupuncture groups, whole blood viscosity (high, medium, low cut), plasma viscosity, red blood cell aggregation index, Evans blue content of brain tissue were all lower than those in model group, the sugar water preference rate, EOF (maximum and minimum) were higher than those in the model group, and the escape latency was shorter than that in the model group, and the number of table crossing was higher than that in the model group, and the differences were statistically significant (P ＜ 0.05). In the low frequency electro-acupuncture group, whole blood viscosity (high, medium, low cut), plasma viscosity, red blood cell aggregation index, Evans blue content of brain tissue were all lower than those in high and medium frequency electro-acupuncture groups, the sugar water preference rate, EOF (maximum and minimum) were higher than those in high and medium frequency electro-acupuncture groups, and the escape latency was shorter than that in the high and medium frequency electro-acupuncture groups, and the number of table crossing was higher than that in high and medium frequency electro-acupuncture groups, and the differences were statistically significant (P ＜ 0.05). There were statistically significant differences in rCBF among model group, high, medium, and low frequency electro-acupuncture groups at each time point (P ＜ 0.05). The rCBF of the model group was lower than that of the sham operation group at each same time point, the rCBF of high, medium, and low frequency electro-acupuncture groups from the first to the fourth week after intervention were higher than those of model group at the time point, the rCBF of low frequency electro-acupuncture group from the first to the fourth week after intervention were higher than those of high and medium frequency electro-acupuncture groups at the same time point, and the differences were statistically significant (P ＜ 0.05). Conclusion Low frequency electroacupuncture can improve the learning and memory ability of cerebral ischemia reperfusion rats. The mechanism may be related to maintaining the integrity of blood-brain barrier function and promoting the recovery of neural function.
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