基于Akt/mTOR信号通路探讨药熨疗法对神经根型颈椎病大鼠神经细胞结构及自噬因子表达的影响

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Abstract Objective To explore the effects of hot medicinal compress therapy on neuronal ultrastructure and expression of autophagy factors in rats with cervical spondylotic radiculopathy (CSR) based on Akt / mTOR signaling pathway. Methods A total of 18 4-week-old SPF SD rats, weighing 150-200 g, and six of them were randomly selected as a blank group, and the rest were established as CSR models. After the molding was successful, they were divided into model group and hot medicinal compress group according to random number table method, with six rats in each group. Blank group and model group were fed normally without any treatment. In the hot medicinal compress group, the bladder channel, the governor channel, and the gallbladder channel were ironed for seven days. After the experiment, the structure of nerve cells was observed by HE staining. Transmission electron microscopy was used to observe the ultrastructure of nerve cells. The expression levels of p-Akt, p-mTOR, Beclin-1, and LC3-Ⅱ/LC3-Ⅰ proteins were detected by Western blot. Results HE staining showed that the tissue morphology of nerve root in the blank group was basically normal. In the model group, the tissue was severely damaged, the outer membrane of nerve root was incomplete, the fibers were disordered, and the axons of some fibers disappeared. The number of cell bodies decreased obviously, but nucleoli were not obvious. In the hot medicinal compress group, the tissue morphology was partially damaged, the nerve root outer membrane was more complete, the fibers were arranged more neatly, and the interstitium was infiltrated with mild inflammatory cells. Transmission electron microscopy showed that cells in blank group had abundant cytoplasm and normal organelles. In the model group, there were nuclear cavities, mitochondrial membrane was fuzzy and some cristae was broken, many lysosomes and autophagosomes were found in the cytoplasm. In the hot medicinal compress group, cytoplasm was abundant, part of mitochondrial membrane was broken, part of cristae was broken and dissolved, lysosomes and autophagosomes were found in cytoplasm. The expression levels of p-Akt and p-mTOR in the model group were lower than those in the blank group, while LC3-Ⅱ/LC3-Ⅰ and Beclin-1 were higher than those in the blank group, the differences were highly statistically significant (P < 0.01). The expression levels of p-Akt and p-mTOR in hot medicial compress group were higher than those in model group, while LC3-Ⅱ/LC3-Ⅰ and Beclin-1 were lower than those in model group, the differences were highly statistically significant (P ＜ 0.01). Conclusion Hot medicinal compress therapy may inhibit excessive autophagy, reduce apoptosis, and promote recovery of damaged nerve roots by enhancing p-Akt and p-mTOR protein expression, and activating Akt / mTOR pathway.

Key words： Akt / mTOR pathway Hot medicinal compress therapy Cervical spondylotic radiculopathy Autophagy

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	ZHAO Yuling HUANG Yi ZHOU Yanqiong JIANG Feifei CHEN Haiyan

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ZHAO Yuling HUANG Yi ZHOU Yanqiong JIANG Feifei CHEN Haiyan. Discussion of effects of hot medicinal compress therapy on nerve cell structure and expression of autophagy factor in rats with cervical spondylotic radiculopathy based on Akt / mTOR pathway#br#[J]. 中国医药导报, 2022, 19(3): 4-8.

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