苦金片对脂多糖诱导巨噬细胞炎症反应的影响

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Abstract Objective To investigate the effect of Kujin Tablets on the inflammatory response and its possible mechanism, the cell model of inflammatory response was established by lipopolysaccharide (LPS) stimulation of mononuclear macrophages in mice. Methods The cells were divided into control group, LPS group and Kujin Tablets group. The cells in LPS group were stimulated with LPS (final concentration of 1 μg/ml) for 12 h, and the Kujin Tablets group was stimulated with Kujin Tablets (1 mg/ml) for 4 h after LPS stimulation. The control group was not treated with special treatment. Interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), transforming growth factor-β (TGF-β), autophagy associated protein 5 (ATG5), microtubule-associated protein 1 light chain 3 Ⅱ(LC3 Ⅱ) mRNA and TNF-α, caspase-3, LC3 Ⅱ, p62 proteins were detected. Results Compared with the control group, the mRNA expression levels of IL-6, TNF-α, TGF-β, ATG5, and LC3 Ⅱ in LPS group were significantly increased, and the differences were statistically significant (P ＜ 0.05). Compared with the control group, the protein expression levels of TNF-α, LC3 Ⅱ, p62 and caspase-3 in LPS group were increased, and the differences were statistically significant (P ＜ 0.05). The mRNA expression levels of IL-6, TNF-α, TGF-β, ATG5, and LC3 Ⅱ in Kujin Tablets group were significantly lower than those in LPS group, the differences were statistically significant (P ＜ 0.05). The protein expression levels of TNF-α, LC3 Ⅱ, p62, and caspase-3 in Kujin Tablets group were significantly lower than those in LPS group, and the differences were statistically significant (P ＜ 0.05). Conclusion Kujin Tablets can significantly reduce LPS-induced cell inflammation, reduce apoptosis and improve cell viability. Autophagy is involved in the anti-inflammatory process of Kujin Tablets.

Key words： Kujin Tablets Lipopolysaccharide Macrophages Inflammation Autophagy

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	LIU Qing ZHU Chunxue LIU Chengyu WANG Yicheng HE Meijuan HE Yanjie HUANG Hanpeng▲

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LIU Qing ZHU Chunxue LIU Chengyu WANG Yicheng HE Meijuan HE Yanjie HUANG Hanpeng▲. Effect of Kujin Tablets on inflammatory response of macrophages induced by lipopolysaccharide#br#[J]. 中国医药导报, 2022, 19(2): 21-24.

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https://www.yiyaodaobao.com.cn/EN/ OR https://www.yiyaodaobao.com.cn/EN/Y2022/V19/I2/21

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