CD47抗体对异丙肾上腺素诱导的大鼠心肌细胞损伤的保护作用

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Abstract Objective To investigate the protective effect of CD47 antibody on Isoproterenol (ISO) induced primary cardiomyocytes injury in rats. Methods The myocardial cells were isolated from 40 SD rats (1-3 days old). The model of primary myocardial cells injury was induced by ISO. The myocardial cells of rats were divided into normal control group (control group), model group (ISO group) and treatment group (Anti-CD47+ISO group) by random number table. In control group, myocardial cells were cultured in the conventional medium; in ISO group, the conventional culture medium was added with ISO (10-5 mol/L) for 48 h; in Anti-CD47+ISO group, CD47 antibody (7 μg/mL) was added to the conventional culture medium for 12 h, followed by ISO (10-5 mol/L) for another 48 h. Cultured supernatants were collected to measure lactic dehydrogenase (LDH), creatine kinase (CK), superoxide dismutase (SOD), malonadehyde (MDA), nitric oxide (NO); the myocardial apoptosis rate was detected using Annexin-V and PI double staining and flow cytometry; dihydroethidium (DHE) staining was used to detecte reactive oxygen species (ROS) in cardiomyocytes; the expression of CD47, Bcl-2 and Bax was detected by Western blot. Results Compared with the control group, the myocardial cells apoptosis rate in ISO group was significantly increased (P < 0.01); compared with the ISO group, the cardiacmyocyte apoptosis rate in Anti-CD47+ISO group was significantly decreased (P < 0.01). Compared with the control group, the levels of LDH, CK, MDA and ROS in ISO group were significantly increased (P < 0.01), the levels of SOD and NO were significantly decreased (P < 0.01); compared with the ISO group, the levels of SOD and NO in Anti-CD47+ISO group were significantly increased (P < 0.01) and the levels of LDH, CK, MDA and ROS were significantly decreased (P < 0.01). The results of Western blot showed that compared with the control group, the expression of CD47 and Bax in ISO group was increased significantly (P < 0.05, P < 0.01), the expression of Bcl-2 in ISO group was decreased significantly (P < 0.01); compared with ISO group, the expression levels of CD47 and Bax in Anti-CD47+ISO group were decreased significantly (P < 0.01); the expression level of Bcl-2 in ISO group was increased significantly (P < 0.05). Conclusion CD47 antibody may inhibit myocardial apoptosis by inhibiting the production of ROS and regulating the expression rate of Bcl-2/Bax protein, thus playing a protective effect on ISO-induced primary myocardial cell injury in rats.

Key words： CD47 antibody Myocardial cells Isoproterenol Anti-oxidant Anti-apoptotic

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	DAI Youjin FU Heling BAO Dan ZHENG Yuan HOU Daorong

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DAI Youjin FU Heling BAO Dan ZHENG Yuan HOU Daorong. The protective effect of CD47 antibody on myocardial cell injury induced by Isoproterenol in rats[J]. 中国医药导报, 2018, 15(26): 13-17,21.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2018/V15/I26/13

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