糖络宁对糖尿病大鼠背根神经节线粒体超氧阴离子产生及线粒体膜电位水平的影响

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Abstract Objective To observe the effect of Chinese herbal compound Tang-luo-ning on oxidative respiratory function of the mitochondrial respiratory chain, superoxide anion production and membrane potential in diabetic rat dorsal root ganglion (DRG). Methods There were 80 SD male rats, 14 of them were randomly selected as the normal group. The rest 66 were used to established diabetic rats model by injected Streptozocin (STZ). According to the random number table, the 61 rats of the model were divided into model group with 19 rats, Western medicine group (alpha lipoic acid group) with 21 rats and traditional Chinese medicine group (Tang-luo-ning group) with 21 rats. They were given the medicine 8 weeks, then mitochondrial respiratory chain oxidative respiratory function, superoxide anion production and membrane potential levels were measured. Results Compared with the normal group, the mitochondrial respiratory function of the DRG in the model group was significantly decreased (P < 0.01). Compared with the model group, the mitochondrial respiratory function of the DRG in the Western medicine group was significantly improved (P < 0.01). Compared with the model group, the Chinese medicine group had no significant improvement in mitochondrial respiratory function in DRG（P ＞ 0.05）. Compared with the normal group, the mitochondrial superoxide anion of the DRG in the model group was significantly increased (P < 0.01). Compared with the model group, the mitochondrial superoxide anion level of the DRG in the Chinese medicine group and the Western medicine group was significantly reduced (P < 0.1). Compared with the normal group, the mitochondrial membrane potential of the model group was significantly decreased (P < 0.01). Compared with the model group, the mitochondrial membrane potential of the Chinese medicine group and the Western medicine group was significantly improved (P < 0.01). Conclusion Tang-luo-ning in hibits the generation of superoxide anion and ameliorates mitochondrial membrane potential in dorsal root ganglions of diabetic rats. This study provides a potential mechanism underlying protection of Tang-luo-ning on the injury of dorsal root ganglions in diabetic model.

Key words： Tang-luo-ning Diabetic rats Oxidative respiratory function Superoxide anion Mitochondria Membrane potential

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	LIU Qin1 LI Yixiao1 ZHANG Taojing2 GONG Yanbing2 ZHOU Hui2 XIE Peifeng2

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LIU Qin1 LI Yixiao1 ZHANG Taojing2 GONG Yanbing2 ZHOU Hui2 XIE Peifeng2. Effect of Tang-luo-ning on mitochondrial superoxide anion production and mitochondrial membrane potential in the dorsal root ganglion of diabetic rats[J]. 中国医药导报, 2018, 15(26): 4-8.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2018/V15/I26/4

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