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| Effect of androgen on inflammatory response of stromal cells in patients with benign prostatic hyperplasia#br# |
| REN Chao YI Faxian HUANG Yong ZHU Xiaojun LI Jianhong |
| Department of Urology, Affiliated Hospital of Inner Mongolia Medicine University, Inner Mongolia Autonomous Region, Hohhot 010050, China |
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Abstract Objective To investigate the effect of Dihydrotestosterone (DHT) on prostatic stromal cell inflammation in aged benign prostatic hyperplasia (BPH) patients. Methods A total of 20 patients who underwent transurethral resection prostate in the Department of Urology, Affiliated Hospital of Inner Mongolia Medical University from January 2016 to May 2017 were collected. Primary prostatic stromal cells were cultured by tissue block method. The expression of Cytokeratin, Desmin, Vimentin and α -smooth muscle actin (α-SMA) in cultured stromal cells of the third generation were determined by immunohistochemical staining. The third generation of cells were cultured in the same size petri dishes and named as blank group, control group and experimental group. Culture medium with the same concentration and dose was added to the blank group. The control group and experimental group were added with tumor necrosis factor-α (TNF-α) 10 ng/ml, 30 nmol/L DHT was added in the experimental group 5 h later. Then co-culture for 24 h, take 100 μl supernatant from three groups, with 4 rewells in each group. The concentrations of interleukin (IL)-6, IL-8, monocyte chemotactic protein-1 (MCP-1) and basic fibroblast growth factor (bFGF) in each group were detected by enzyme-linked immunosorbent assay. Results Primary prostatic interstitial cells were cultured in vitro. Cytokeratin and Desmin were negatively expressed in the third generation of prostatic interbasal cells. Vimentin and α-SMA were positive. The concentrations of IL-6, IL-8, MCP-1, and bFGF in the control group were higher than those in the blank group, the differences were statistically significant (P ＜ 0.05), while the concentrations of IL-6, IL-8, MCP-1 and bFGF in the experimental group were lower than those in the control group, the differences were statistically significant (P ＜ 0.05). Conclusion DHT has a direct anti-inflammatory effect on prostatic interstitial cells in aged patients with prostatic hyperplasia.
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