Molecular mechanism exploration of Semen Euryales-Fructus Rosae Laevigatae in the treatment of diabetic nephropathy based on network pharmacology#br#
LI Xiaoran1 WANG Xueying2 ZHANG Hua1 WANG Shidong1 ZHENG Xiaoyan1
1.The Second Department of Nephropathy and Endocrinology, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China; 2.Department of Geratology, Beijing Electric Power Hospital, Beijing 100073, China
Abstract Objective To investigate the molecular mechanism of Semen Euryales-Fructus Rosae Laevigatae in the treatment of diabetic nephropathy (DN) based on network pharmacology. Methods The drug ingredients and targets were investigated by traditional Chinese medicine systems pharmacology database and analysis platform, encyclopaedia of traditional Chinese medicine, and Swiss Target Prediction databases, and the GeneCards and OMIM databases were searched for targets related to DN. The protein-protein interaction network and drug-target network were constructed by STRING platform and Cytoscape software, and network topology analysis and core target screening were completed. The enrichment analyses of gene ontology and Kyoto Encyclopedia of Genes and Genomes were completed by R software, and the core pathways and targets were obtained. Finally, the molecular docking of core ingredient targets was completed by VINA software. Results Two active ingredients of Semen Euryales, and seven active ingredients of Fructus Rosae Laevigatae were obtained, including quercetin, kaempferol, gondoic acid, and other core active ingredients. The 20 core targets including mitogen activated protein kinase (MAPK) 1, and transcription factor-AP1 (Jun), MAPK3 were screened. Gene ontology enrichment analysis showed that the targets were mainly concentrated in the cell membrane region, which played various biological functions including cell signal transduction and nuclear transcription, and completed multiple regulatory response to lipopolysaccharide and oxidative stress. Kyoto Encyclopedia of Genes and Genomes enrichment analysis showed that the main enrichment pathways were the AGE-RAGE, MAPK, PI3k-Akt, NF-κB, and other signaling pathways. The results of molecular docking showed that quercetin and kaempferol had good affinity with core targets, such as MAPK1, transcription factor-AP1 (Jun) and so on. Conclusion This study applies the network pharmacology method to elucidate the possible mechanism of Semen Euryales-Fructus Rosae Laevigatae pairs in the treatment of DN, including multiple pathways and targets to regulate the complex inflammatory response and oxidative stress, which provides clues and basis for further experimental and clinical pharmacological studies.
LI Xiaoran1 WANG Xueying2 ZHANG Hua1 WANG Shidong1 ZHENG Xiaoyan1. Molecular mechanism exploration of Semen Euryales-Fructus Rosae Laevigatae in the treatment of diabetic nephropathy based on network pharmacology#br#[J]. 中国医药导报, 2021, 18(36): 34-39.
