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| Effect of miR-189 on bone formation and its mechanism#br# |
| LIU Xingyu1 WANG Jie2 SHI Fei3 |
1.Department of General Internal Medicine, Jingzhong Medical District, PLA General Hospital, Beijing 100011, China;
2.Department of Endocrinology and Metabolism, Xi’an Daxing Hospital, Shaanxi Province, Xi’an 710000, China;
3.School of Aeronautics and Astronautics, Air Force Medical University, Shaanxi Province, Xi’an 710032, China |
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Abstract Objective To explore the effect and mechanism of miR-189 on bone formation. Methods Thirty-two female C57BL/6J mice were divided into four groups by random number table method: sham operation group, castration group, control group, and inhibitor group, with eight mice in each group. Ovarian castration was used to construct a mouse model of osteoporosis. The content of miR-189 in femur was detected by RT-qPCR. After injection of miR-189 negative control sequence (control group) or inhibitor (inhibitor group) into tail vein of emasculated mice, bone formation was detected by micro CT and calcein staining. MC3T3-E1 was transfected with miR-189 negative control sequence, mimics (mimics group) or inhibitors. Western blot was used to detect osteogenic indexes. Results Compared with sham operation group, miR-189 expression of femur in castration group was higher (P ＜ 0.05). Compared with control group, miR-189 of femur in inhibitor group was decreased (P ＜ 0.05), and the bone loss caused by castration was partially alleviated (P ＜ 0.05). Compared with control group, miR-189 was increased in mimics group (P ＜ 0.05), miR-189 was decreased in inhibitor group (P ＜ 0.05), compared with the control group, the osteogenic indexes in the mimics group were decreased (P ＜ 0.05), and the osteoblast index in inhibitor group increased (P ＜ 0.05). Conclusion The femur of emasculated mice is highly expressed with miR-189, which inhibits bone formation by inhibiting osteoblast function.
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