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| Effect of electroacupuncture on the expression of NADPH oxidase in the substantia nigra of Parkinson disease mice induced by LPS#br# |
| KANG Kaiwen1 YIN Faming2 WANG Yuan3 YUAN Wei3 LU Gang3 BAI Qiuju1 WANG Qiang3 |
1.The Second Clinical Medical College, Shaanxi University of Chinese Medicine, Shaanxi Province, Xianyang 712046, China;
2.Department of Rehabilitation, Qingdao Hospital of Traditional Chinese Medicine, Shandong Province, Qingdao 266000, China;
3.College of Acupuncture and Massage, Shaanxi University of Chinese Medicine, Shaanxi Province, Xianyang 712046, China |
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Abstract Objective To investigate the effect of electroacupuncture on the expression of p47phox and p22phox of reduced nicotinamide adenine dinucleotide phosphate (NADPH) oxidase subunits in the substania nigra of lipopolysaccharide (LPS) induced Parkinson disease (PD) mice, and to explore the mechanism of electroacupuncture in improving PD. Methods Forty male C57BL/6 mice with clean grade of 22-25 g were divided into blank group, model group, Levodopa group, and electroacupuncture group by random number table method, with ten mice in each group. Except blank group, the other groups were given 10 μl (1 g/L, dissolved in the 0.9% sodium chloride solution) LPS by nasal drip to make PD model. The blank group was given the same amount of the 0.9% sodium chloride solution. Levodopa group was intraperitoneally injected with 0.2 ml Levodopa Injection (10 mg/kg). Electroacupuncture group acupuncture “yìntáng” “yíngxiāng” point (2 Hz/100 Hz, 1 mA, 20 min). Blank group and model group were captured in the same degree. After the intervention, the mice were subjected to behavioral testing, and the expression of p47phox and p22phox in substantia nigra cells was detected by immunohistochemical method. Results Compared with blank group, the total number of arm advances and accuracy in model group was decreased significantly (P ＜ 0.01). Compared with model group, the total number of arm entry and accuracy of electroacupuncture group was increased (P ＜ 0.05). Compared with blank group, the number of p47phox and p22phox protein positive cells in model group was increased significantly (P ＜ 0.01). Compared with model group, the number of p47phox and p22phox protein positive cells in the substantia nigra in electroacupuncture group was decreased (P ＜ 0.05). Conclusion “Xiu Sanzhen” can inhibit oxidative stress, improve motor symptoms and delay PD process in PD mice, which may be related to the inhibition of NADPH oxidase.
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