基于高通量测序筛选lncRNAs在宫颈鳞状细胞癌中的表达及临床意义

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Abstract Objective To investigate the expression and clinical significance of long non-coding RNA (TOPORS-AS1, TMPO-AS1, LINC00925, MIR155HG, and NR2F2-AS1) in cervical squamous cell carcinoma (CSCC) based on high-throughput sequencing. Methods Cervical tissues of 80 Uyghur patients admitted to the First Affiliated Hospital of Xinjiang Medical University from June to December 2019 were collected, including cervicitis (20 cases), high-grade squamous intraepithelial lesion (HSIL, 20 cases) and CSCC (40 cases). The relative expression levels of long non-coding RNA in each group were detected, the differences of long non-coding RNA in each group and the relationship between MIR155HG and clinicopathological features of CSCC patients were compared. Results The expressions of TMPO-AS1, LINC00925, MIR155HG, and NR2F2-AS1 in CSCC group were higher than those in cervicitis group, and the differences were statistically significant (P < 0.05); the expressions of TOPORS-AS1, TMPO-AS1, LINC00925, MIR155HG, and NR2F2-AS1 in CSCC group were higher than those in HSIL group, and the differences were statistically significant (P < 0.05). The expression of MIR155HG in CSCC patients with mass diameter > 4 cm was higher than that in patients with mass diameter ≤4 cm, and the expression of MIR155HG in lymph node metastasis was higher than that in patients without lymph node metastasis, and the differences were statistically significant (P < 0.05). Conclusion TOPORS-AS1, TMPO-AS1, LINC00925, MIR155HG, and NR2F2-AS1 are highly expressed in cervical cancer tissues, and the expression of MIR155HG is correlated with mass diameter and lymph node metastasis status.

Key words： Cervical cancer Long non-coding RNA Clinical feature

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	CHEN Yanxia LIANG Lingyun CHEN Bing MA Cailing▲

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CHEN Yanxia LIANG Lingyun CHEN Bing MA Cailing▲. Expression and clinical significance of screen lncRNAs in cervical squamous cell carcinoma based on high-throughput sequencing#br#[J]. 中国医药导报, 2021, 18(35): 101-104.

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https://www.yiyaodaobao.com.cn/EN/ OR https://www.yiyaodaobao.com.cn/EN/Y2021/V18/I35/101

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