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| Effects of Chinese medicine barbary wolfberry fruit and danshen root for tonifying deficiency and promoting blood circulation on the expression of TNF-α and IL-1β in the retina of rd10 mice#br# |
| JIANG Pengfei1,2 YAO Zhen1,2▲ OU Chen1,2 YANG Yijing1,2 PENG Jun1,3 XU Jian4 PENG Qinghua1,2▲ |
1.College of Traditional Chinese Medicine, Hunan University of Chinese Medicine, Hunan Province, Changsha 410208, China;
2.Hunan Engineering Technology Research Center for the Prevention and Treatment of Otorhinolaryngologic Diseases and Protection of Visual Function with Chinese Medicine, Hunan Province, Changsha 410208, China;
3.Department of Ophthalmology, the First Hospital of Hunan University of Chinese Medicine, Hunan Province, Changsha 410007, China;
4.Department of Ophthalmology, Shanghai Oriental Hospital, Shanghai 200120, China |
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Abstract Objective To observe the effects of Chinese medicine barbary wolfberry fruit and danshen root for tonifying deficiency and promoting blood circulation on the expression of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) in the retina of rd10 mice. Methods Forty four-week-old rd10 (Pde6b, -/-) mice with the body weight of 18-20 g were divided into model group, control group, barbary wolfberry fruit group, danshen root group, and barbary wolfberry fruit with danshen root group according to random number table method, with eight mice in each group. Physiological saline, vitamin A aqueous solution, Barbary Wolfberry Fruit Chinese medicine Formula Granules aqueous solution, Danshen Root Chinese medicine Formula Granules aqueous solution, Barbary Wolfberry Fruit and Danshen Root Chinese medicine Formula Granules aqueous solution were given 20 ml/(kg·d) by gavage, respectively. Eight C57 wild-type (Pde6b, +/+) mice were given the same amount of normal saline by gavage. The samples were taken after 28 days of gavage. The expression levels of TNF-α and IL-1β in the retina of mice in each group were detected by real-time fluorescence quantitative PCR and Western blot. Results Compared with blank group, the protein and mRNA relative expression levels of TNF-α and IL-1β in model group were increased (P ＜ 0.05). Compared with model group, the protein and mRNA relative expression levels of TNF-α and IL-1β in barbary wolfberry fruit group, danshen root group, and barbary wolfberry fruit with danshen root group were decreased (P ＜ 0.05). Compared with barbary wolfberry fruit group, the relative expression levels of TNF-α and IL-1β protein and IL-1β mRNA in barbary wolfberry fruit with danshen root group were decreased (P ＜ 0.05). Compared with danshen root group, the protein and mRNA relative expression levels of TNF-α and IL-1β in barbary wolfberry fruit with danshen root group were decreased (P ＜ 0.05). Conclusion Chinese medicine for tonifying deficiency and promoting blood circulation, Chinese medicine barbary wolfberry fruit and danshen root can reduce the expression levels of TNF-α and IL-1β in the retina of rd10 mice, and protect the ultrastructure of the retina.
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[1] Ikeda HO，Sasaoka N，Koike M，et al. Novel VCP modulators mitigate major pathologies of rd10，a mouse model of retinitis pigmentosa [J]. Sci Rep，2014，4（1）：1-9.
[2] Donato L，Scimone C，Alibrandi S，et al. Discovery of GLO1 new related genes and pathways by RNA-Seq on A2E-stressed retinal epithelial cells could improve knowledge on retinitis pigmentosa [J]. Antioxidants，2020，9（5）：416.
[3] Caruso S，Ryu J，Quinn PMJ，et al. Precision metabolome reprogramming for imprecision therapeutics in retinitis pigmentosa [J]. J Clin Invest，2020，130（8）：3971-3973.
[4] Wierzba-Bobrowicz T，Schmidt-Sidor B，Gwiazda E，et al. Major histocompatibility complex class Ⅱ expression in the frontal and temporal lobes in the human fetus during development [J]. Folia Neuropathol，2000，38（2）：73-77.
[5] Makabe K，Sugita S，Mandai M，et al. Microglia dynamics in retinitis pigmentosa model： Formation of fundus whitening and autofluorescence as an indicator of activity of retinal degeneration [J]. Sci Rep，2020，10（1）：14700.
[6] Murakami Y，Nakabeppu Y，Sonoda KH. Oxidative Stress and Microglial Response in Retinitis Pigmentosa [J]. Int J Mol Sci，2020，21（19）：7170.
[7] 韦道祥.小胶质细胞与阿尔茨海默病[J].国际老年医学杂志，2000，21（6）：248-250.
[8] Massengill MT，Ash NF，Young BM，et al. Sectoral activation of glia in an inducible mouse model of autosomal dominant retinitis pigmentosa [J]. Sci Rep，2020，10（1）：16967.
[9] Yang W，Xiong G，Lin B. Cyclooxygenase-1 mediates neuroinflammation and neurotoxicity in a mouse model of retinitis pigmentosa [J]. J Neuroinflamm，2020，17（1）：1-17.
[10] Terauchi R，Kohno H，Watanabe S，et al. Minocycline decreases CCR2-positive monocytes in the retina and ameliorates photoreceptor degeneration in a mouse model of retinitis pigmentosa [J]. PLoS One，2021，16（4）：e0239108.
[11] 刘家琪，王英，蒋鹏飞，等.枸杞丹参对视网膜色素变性大鼠视网膜组织形态学及CRYAB mRNA的影响[J].中国医药导报，2019，16（14）：13-15.
[12] 蒋鹏飞，王英，潘坤，等.益气明目丸对视网膜色素变性大鼠视网膜Fas、FasL蛋白表达的影响[J].中医杂志，2019，60（4）：62-67.
[13] 蒋鹏飞，欧晨，彭俊，等.枸杞子-丹参对小鼠视网膜Caspase-12前体、Caspase-2表达的影响[J].湖南中医药大学学报，2021，41（4）：504-511.
[14] 欧晨，宋厚盼，杨毅敬，等.基于网络药理学探讨枸杞子治疗视网膜色素变性的作用机制[J].世界中医药，2021， 16（8）：1192-1197.
[15] 欧晨，彭俊，蒋鹏飞，等.枸杞丹参对rd10小鼠感光细胞凋亡及氧化应激的影响[J].时珍国医国药，2020，31（11）：2580-2582.
[16] 彭俊，蒋鹏飞，王英，等.枸杞子加丹参对RCS（rdy-/-，p-/-）大鼠视网膜组织形态及感光细胞凋亡的影响[J].湖南中医药大学学报，2020，40（9）：1086-1089.
[17] Xu J，Yang YJ, Qin GY，et al. Effects of Lycii Fructus and Salviae Miltiorrhizae on the Syndrome of Deficiency with Blood Stasis in RCS （rdy-/-，p-/-）Rats with Retinitis Pigmentosa：An Intervention Study [J]. Digital Chinese Medicine，2019，2（3）：157-165.
[18] 俞洋，冯振娥，李楠.枸杞子含药血清对兔视网膜色素上皮细胞光损伤保护的实验研究[J].中华中医药杂志，2012，27（11）：2950-2952.
[19] 徐剑，周亚莎，彭俊，等.枸杞子加丹参对RCS（rdy-/-，p-/-）大鼠视网膜匀浆中cAMP、cGMP含量的影响[J].湖南中医药大学学报，2020，40（7）：784-787.
[20] 蒋鹏飞，彭俊，欧晨，等.视网膜色素变性的实验研究进展[J].国际眼科杂志，2020，20（6）：970-973.
[21] 彭俊，王英，蒋鹏飞，等.枸杞丹参对RCS大鼠视网膜组织中Bcl-2、Bid表达的影响[J].世界科学技术-中医药现代化，2020，22（4）：1166-1175.
[22] 宋厚盼，曾梅艳，陈小娟，等.枸杞子促进虹膜色素上皮细胞定向分化治疗视网膜色素变性的分子网络调控机制研究[J].中国临床药理学与治疗学，2019，24（12）：1402-1414.
[23] 刘丽娅，马景学，安建斌，等.姜黄素、丹参单体和苦参碱抑制IL-1β诱导下兔RPE细细胞增生的体外实验研究[J].中华实验眼科杂志，2016，34（8）：699-704.
[24] 倪颖勤，姜春晖，徐格致，等.大鼠视网膜光损伤模型中小胶质细胞的迁移及活化[J].复旦学报：医学版，2011， 38（2）：126-130.
[25] 曾惠阳，曹安民，朱秀安.小胶质细胞在人原发性视网膜色素变性中的活化[J].中华实验眼科杂志，2008，26（2）：129-132. |
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