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Exploration on the mechanism of Astragali Radix and Codonopsis Radix couplet medicines in treating diabetic nephropathy based on network pharmacology#br# |
LIU Zhengping1 CAO Po2 ZHONG Jian2 WEI Yu2 WANG Shidong2 SHI Yuexin1 |
1.The First Clinical Medical College, Beijing University of Chinese Medicine, Beijing 100700, China;
2.Department of Nephropathy, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China |
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Abstract Objective To explore the mechanism of Astragali Radix and Codonopsis Radix couplet medicines in treating diabetic nephropathy based on network pharmacology. Methods Traditional Chinese medicine systems pharmacology database and analysis platform was used to search for the effective components and related targets of Astragali Radix and Codonopsis Radix. The network diagram of drug effective components-target was plotted by using Cytoscape 3.8.1 software. OMIM, DisGeNET and GeneCards databases were used to obtain related targets of diabetic nephropathy. The intersections with drug targets were uploaded by using STRING 11.0 database to construct a protein protein interaction network. Metascape database was used for gene ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis on the intersecting targets. Results A total of 34 effective components were obtained from Astragali Radix and Codonopsis Radix couplet medicines, and 15 core targets were obtained from the intersecting targets. KEGG analysis showed that its main pathways of intersection targets included signaling pathways of AGE-RAGE, IL-17, MAPK, NF-κB, VEGF, JAK-STAT, and so on. Conclusion Astragali Radix and Codonopsis Radix couplet medicines may have effect on diabetic nephropathy through the action of quercetin, kaempferol, luteolin and other components on multiple targets such as AKT1, IL-6, TNF, and the regulation of multiple signaling pathways such as AGE-RAGE, IL-17, and others.
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