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| Association of TRPV1 gene polymorphism with opioid requirements in patients with advanced lung cancer |
| WANG Dan1 WU Chengfeng1 SHEN Wen2▲ |
1.Department of Anesthesiology, Graduate School of Xuzhou Medical University, Jiangsu Province, Xuzhou 221004, China;
2.Department of Pain, Affiliated Hospital of Xuzhou Medical University, Jiangsu Province, Xuzhou 221004, China |
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Abstract Objective To investigate the relationship between transient receptor potential vanilloid 1 (TRPV1) gene polymorphisms and opioid requirements in patients with advanced lung cancer. Methods Peripheral venous blood samples were collected from 191 patients with advanced lung cancer (stages Ⅲ and Ⅳ) (lung cancer group) and 52 healthy controls (healthy control group) in Affiliated Hospital of Xuzhou Medical University from December 2016 to May 2017. The genotypes of rs222747 and rs8065080 of TRPV1 were analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Part of samples were randomly selected and verified by direct sequencing method. Visual analogue scale (VAS) was used to evaluate the pain of patients and morphine equivalents were used to compare the requirements of opioids for patients with different genotypes. Results Three genotypes were detected in lung cancer group and healthy control group: rs222747 (CC, GC, GG) and rs8065080 (CC, TC, TT). There was no significant difference in the genotype and allele distribution of rs222747 between the lung cancer group and healthy control group (P > 0.05). The distribution of genotypes and alleles of the rs8065080 between the lung cancer group and the healthy control group were statistically significant(P < 0.05). There was no significant difference in the 24 h-opioids doses, weight-adjusted-24 h-opioids doses, weight-surface area-adjusted-24 h-opioids doses between the genotypes and alleles of rs222747 in the lung cancer group with pain(P > 0.05). Conclusion There is insufficient evidence that the rs222747 polymorphisms of TRPV1 are associated with the requirement of opioids in advanced lung cancer patients with pain.
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