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| Effects of alcohol extract of Waternut Herb on behavior and hippocampal inflammatory media in mice with Alzheimer disease |
| ZHONG Lili1，2 ZHANG Weijia3 LIU Xu4 SUN Zhongren5 |
1.Department of Pathology, the First Affiliated Hospital of Heilongjiang University of Chinese Medicine, Heilongjiang Province, Harbin 150040, China;
2.Postdoctoral Station, Heilongjiang University of Chinese Medicine, Heilongjiang Province, Harbin 150040, China;
3.Department of Orthopedics, the First Hospital of Harbin City, Heilongjiang Province, Harbin 150010, China;
4.Experiment Center, Heilongjiang University of Chinese Medicine, Heilongjiang Province, Harbin 150040, China;
5.Laboratory of Acupuncture Clinical (Encephalopathy) Neurobiology, Heilongjiang University of Chinese Medicine, Heilongjiang Province, Harbin 150040, China |
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Abstract Objective To investigate the effects of alcohol extract of Waternut Herb on learning ability, memory ability, learning and memory retention ability of mice with Alzheimer disease, and the effects of interleukin (IL)-1β and tumor necrosis factor-α (TNF-α) in hippocampal inflammation media. Methods Forty-eight male C57BL/6 mice were divided into six groups according to the random number table method: model group, normal group, Donepezil group, and high, medium, low dose of alcohol extract of Waternut Herb group, with 8 mice in each group. Alzheimer disease model was induced by bilateral intracere broventricular injection of Aβ1-42 (80 pmol/μL). Each group of rats was given intragastric administration once a day. The mice in Donepezil group were given 0.000 92 g/(kg·d) by gavage. The mice in the high, medium, low dose of alcohol extract of Waternut Herb group were given 3.96, 1.98, 0.99 g/(kg·d) by gavage, respectively. While the mice in the normal group and model group were given intragastric administration with continuous saline, treatment for 35 days. The Morris water maze was used to detect the changes of behavior in Alzheimer disease mice. The levels of IL-1β and TNF-α in hippocampus of Alzheimer disease mice were determined by enzyme-linked immunosorbent assay. Results The incubation period and swimming distance of mice in the model group were increased. At the same time, the target residence time was shortened and the number of cross-platforms was reduced, it took a long time to find the underwater platform of the water maze system, compared with the normal group, the abnormal behavior in the model group was significantly increased (P ＜ 0.05). Compared with the normal group, the levels of IL-1β and TNF-α of the inflammatory mediators of the hippocampus in model group were significantly increased (P ＜ 0.05). Compared with the model group, the behavior of mice in the high and medium doses of alcohol extract of Waternut Herb group was significantly improved, and the levels of IL-1β and TNF-α in the hippocampus were significantly decreased, the differences were statistically significant (P ＜ 0.05). Conclusion Alcohol extract of Waternut Herb can improve the learning ability and memory impairment of mice, reduce the inflammatory response and relieve blood-brain barrier injury, and plays an important role in the treatment of Alzheimer disease.
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