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| Effect of moxibustion on wound repair in rats with pressure injuries |
| YU Jie1 SUN Zhongren2 LI Hongling3 ZHAO Hui2 |
1.Affiliated Hospital of Yangzhou University, Jiangsu Province, Yangzhou 225000, China;
2.Heilongjiang University of Chinese Medicine, Heilongjiang Province, Harbin 150040, China;
3.LI Hongling Surgical Clinic of Traditional Chinese Medicine, Heilongjiang Province, Harbin 150030, China |
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Abstract Objective To observe the effect of moxibustion on wound repair in rats with pressure injuries. Methods The models of rats with pressure ulcers which belonged to stage 2-3 were established by using self-made devices of pressure ulcers. The models of 70 SD female rats with skin ischemia reperfusion injury were randomly divided into model group and moxibustion group. There were 35 rats in each group. Each group was divided into 5 subgroups including 1 d, 3 d, 5 d, 7 d and 10 d according to the length of intervention time. Seven experimental rats were included in each subgroup. The blank group was given shaving disinfection, tied together with other groups of rats given only conventional iodophor intervention. The model group was only given conventional iodophor intervention. The moxibustion group was given iodophor intervention at first and direct moxibustion intervention to the local tissue of pressure ulcer with cyclotron operation, with moxibustion once a day and 15 minutes each time. The wound area was measured respectively after five time-points (1 d, 3 d, 5 d, 7 d and 10 d after intervention or treatment) before drawing. HE staining was used to observe the pathological changes of the pressure ulcer. Results There were no significant differences in the wound healing rates between the groups in the 1 d after intervention(P > 0.05). Compared with the model group, the wound healing rates of moxibustion group were significantly higher at the four time-points including 3 d, 5 d, 7 d and 10 d after treatment. At the same time-points, there were significant differences between the two groups (P < 0.01). Compared with the model group, the granulation tissues in the wounds of rats which belonged to moxibustion group with pressure ulcers had obvious advantages both in the time-points and the growth states. There were granulation tissues at the third day after treatment in the moxibustion group, while those of model group appeared about at the seventh day after iodophor intervention. Simultaneously, the wound contraction rates of pressure ulcers were faster in the moxibustion group which had more evident area reductions. Pathological changes of pressure ulcers were observed by HE staining that the peaks of the inflammatory infiltrations which existed in the moxibustion group had appeared in advance compared with the model group. Meanwhile, fibroblasts appeared earlier, having more numbers and obvious degrees of hyperplasia. The occurrence time of granulation tissues was earlier in the moxibustion group. Hence, neovascularization which belonged to moxibustion group formed earlier than that of model group. The epidermal structures of rats in the moxibustion group recovered in advance. Conclusion Moxibustion can promote the wound healing effectively in rats with pressure injuries.
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[1] （意）罗曼艾里.压疮诊疗新进展与实践[M].北京：人民军医出版社，2013.
[2] Gordon MD，Gottschlich MM，Helvig EI，et al. Review of evidenced-based practice for the prevention of pressure ulcers in burn patients [J]. J Burn Care Res，2004，25（5）：388-410.
[3] Anthony D，Reynolds T，Russell L. an investigation into the use of serum albumin in pressure sore prediction [J]. J Adv Nurs，2000，32（2）：359-365.
[4] 邓欣，吕娟，陈佳丽，等.2016年最新压疮指南解读[J].华西医学，2016，31（9）：1496-1498.
[5] Sun ZR，Yue JH，Zhang QH. Electroacupuncture at Jing-jiaji points for neck pain caused by cervical spondylosis：a study protocol for a randomized controlled pilot trial [J]. Trials，2013，14（1）：360.
[6] 于杰，孙忠人，李洪玲，等.艾灸治疗压疮的研究现状[J].现代生物医学进展，2017，17（6）：1183-1186.
[7] 于杰，孙忠人，李洪玲，等.对大鼠压疮缺血-再灌注损伤模型构建及应用的思考[J].现代生物医学进展，2017，17（7）：1210-1214.
[8] 于杰.基于RAS/RAF/ERK信号通路探讨艾灸促进压疮组织血管新生的分子调控机制研究[D].哈尔滨：黑龙江中医药大学，2017.
[9] 于杰，孙忠人，李洪玲，等.艾灸对压疮大鼠创面局部平均血流灌注量的影响[J].辽宁中医药大学学报，2018，20（11）：8-14.
[10] Zhang Q，Sun Z，Yue J，et al. Traditional Chinese Medicine for Pressure Ulcer：A Meta-Analysis [J]. Int Wound J，2013， 10（2）：221-231.
[11] Ren X，Sun ZR，Zhang QH，et al. Nursing of Pressure Ulcers：A Systematic Review [J]. Clin J Prac Nurs，2011，27（6）：1-4.
[12] 仇立波，孙忠人，张秦宏，等.电针傍刺对压疮大鼠皮肤p38MAPK表达的影响[J].中医药学报，2016，44（1）：54-57.
[13] 郑敏，甘秀妮.局部氧疗联合封闭式负压引流对豚鼠Ⅲ期压疮创面愈合的影响[J].上海交通大学学报：医学版，2012，32（7）：911-916.
[14] 洪文学，蔡建红，景军.艾灸的热辐射光谱特性研究[J].应用光学，2004，25（4）：1-3.
[15] 邓海平，沈雪勇.艾灸与经络穴位红外辐射特性[J].中国针灸，2004，24（2）：105-107.
[16] 杨华元，刘堂义.艾灸疗法的生物物理机制初探[J].中国针灸，1996，16（10）：17-18.
[17] Yang HY，Hu ZC. Biophysical characteristics of moxibustion [J]. WJAM，2010，30（2）：27-37.
[18] 许培昌，李达良，崔淑丽.不同施灸距离对人体体表皮肤温度的影响—论施灸的安全距离[J].中国针灸，2012， 32（7）：611-614.
[19] Herndon ND. Total Burn Care [M]. New York：Harcourt Publishers Limited，2002：516.
[20] Greenhalgh DG，Lawless MB，Chew BB，et al. Temperature threshold for burn injury：an oximeter safety study [J]. J Burn Care Rehabil，2004，25（5）：411-415.
[21] 施睿，邱悦，余芝，等.不同温度艾灸对施灸局部微循环影响的实验观察[J].辽宁中医杂志，2016，43（5）：1092-1094.
[22] 高希言，陈岩，王鑫，等.腹部透灸时温度变化的研究[J].中国针灸，2015，35（1）：45-49. |
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