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| Changes of renin-angiotensin in rats with cerebral-cardiac syndrome after subarachnoid hemorrhage |
| PAN Rui1 DU Yeliang2▲ MU Weinan1 LIU Qihui1 ZHAO Yu1 |
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Abstract 1.Graduate School, Weifang Medical University, Shandong Province, Weifang 261000, China; 2.Department of Neurology, Brain Hospital, Weifang People′s Hospital, Shandong Province, Weifang 261000, China
[Abstract] Objective To investigate the changes of renin-angiotensin in rats with subarachnoid hemorrhage (SAH) complicated by cerebral-cardiac syndrome (CCS). Methods The occipital large pool was injected with autologous arterial blood to replicate the SAH model. Seventy-five healthy male Wistar rats were 6 weeks old and weighed 150-200 g. All rats were connected to a dynamic laboratory system (Power Lab system) to detect ECG, excluding abnormal ECG. The rats were randomly divided into surgery group (50 rats) and sham group (25 rats). The surgical group was divided into SAH group and CCS group according to different electrocardiograms after surgery. Each group had 25 animals (insufficient ones were recruited in the same condition as new rat). Different time periods were randomly divided into five subgroups: 1, 3, 5, 7, 14 d group, with 5 rats in each group. Electrocardiograms were measured with the Power Lab system after modeling, and electrocardiograms were again detected on day 1, 3, 5, 7 and 14. The levels of plasma renin activity (PRA) and angiotensin Ⅱ (AngⅡ) were detected by enzyme-linked immunosorbent assay (ELISA). Results After successful modeling, there was no significant change in electrocardiogram in the sham-operated group. The incidence of cerebral-cardiac syndrome was highest in the surgical group at 2 hours after surgery. The levels of PRA and AngⅡ in SAH group and CCS group were higher than those in sham-operated group at day 1 (P ＜ 0.05), and the CCS group was higher than the SAH group (P ＜ 0.05); PRA and AngⅡ peaked at 3 days in SAH group and CCS group were significantly higher than that in sham operation group (P ＜ 0.05), and CCS group was higher than the SAH group (P ＜ 0.05). The contents of PRA and AngⅡ in SAH group and CCS group decreased gradually after 5 and 7 days, which was still higher than that in sham operation group (P ＜ 0.05). The contents of PRA and AngⅡ gradually decreased to 7 days and then stabilized in the sham operation group. The levels of PRA and AngⅡ in the SAH group and the CCS group reached the peak at 3 days, and then gradually decreased to 14 days afterwards, and the levels of PRA and AngⅡ basically reached the level of the sham operation group (P ＞ 0.05). Conclusion PRA and AngⅡ in SAH complicated with CCS gradually increase and reach a peak, then gradually decrease and tend to be stable. This suggests that PRA and AngⅡ play a certain role in the occurrence and development of SAH concurrent with CCS.
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