miR-221对结核分枝杆菌感染巨噬细胞后炎性因子表达的影响

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Abstract Objective To explore the effects of miR-221 on the inflammatory factor secretion from macrophages after Mycobacterium tuberculosis infection. Methods The expression of miR-221 in macrophages after Mycobacterium tuberculosis infection was detected by real-time quantitative PCR (qRT-PCR). Macrophages were transiently transfected with miR-221 mimic or miR-221 inhibitor. The expression of macrophage inflammatory factor and secretion of NO were detected by enzyme-linked immunosorbent assay (ELISA) and Griess method, respectively. The relationship of miR-221 and Rho-associated, coiled-coil containing protein kinase 1 (ROCK1) was analyzed by dual-luciferase reporter gene assay, qRT-PCR and Western blot. Results Down-regulated miR-221 was observed in macrophages infected with Mycobacterium tuberculosis. The expression of miR-221 in macrophages was up-regulated by miR-221 mimics, but down-regulated by miR-221 inhibitors. The secretions of TNF-α, IL-1β, IL-6 and NO were significantly accelerated in macrophages infected with Mycobacterium tuberculosis, and increased in miR-221 up-regulated macrophages, but suppressed in miR-221 down-regulated macrophages. miR-221 directly binds to the 3′UTR of ROCK1, and negatively regulated its expression. Conclusion miR-221 can regulate the ROCK1 expression and inhibit the secration of inflammatory factors expression in macrophages after Mycobacterium tuberculosis infection.

Key words： Mycobacterium tuberculosis Monocyte-derived macrophages MicroRNA-221 Rho-associated, coiled-coil containing protein kinase 1 Inflammatory factors

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	HE Baoming BAI Ying LI Yanqin

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HE Baoming BAI Ying LI Yanqin. Effects of miR-221 on the inflammatory factor secretion from macrophages after Mycobacterium tuberculosis infection[J]. 中国医药导报, 2018, 15(24): 14-17,29.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2018/V15/I24/14

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