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| Experimental study on toxicology related to nanomagnetic beads technology |
| WANG Xin ZHANG Liming |
| Department of Internal Neurology, the First Affiliated Hospital of Harbin Medical University, Heilongjiang Province, Harbin 150000, China |
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Abstract Objective To study the toxicology of nanomagnetic beads and to understand the distribution of metabolism in rats. Methods Twenty-four healthy adult rats were randomly divided into 8 groups with 3 rats in each group. One group without any treatment as a blank control group, one group was injected with normal saline as positive control group, the other 6 groups were injected with nano-oxidized iron magnetic beads as the experimental group, the growth, behavior and neurological changes of the rats of each group were observed. HE staining and Prussian blue iron staining were used to observe the changes of organ structure and local pathological changes such as inflammatory reaction of rats in the experimental groups, and the distribution of magnetic beads in the main organs of the rats. Ten rats were randomly divided into two groups, and with 5 rats in each group, rats in the two groups were injected with ferromagnetic beads or normal saline respectively, and the cognitive function of rats was observed. Blood of 4 healthy New Zealand rabbits were collected after the injection of iron oxide magnetic beads and changes of related indexes were observed. Results There was no abnormal growth or appearance in the experimental group and the blank control group. The Longa score was 0, no abnormal changes were observed. Water maze experiments of the two groups including the escape latency and the number of crossing platforms were scored, there was no statistically significant (P > 0.05). There were no structural and pathological changes in the organ morphology of the rats in each group. The iron oxide magnetic beads had left the circulatory system and entered various organs of the rats after being injected for 5 minutes. Most of the magnetic beads were distributed in the spleen and liver. The difference in creatinine levels at different time points after injecting iron oxide magnetic beads in rabbits was statistically significant (P < 0.05), but still in the normal range. Other serum biochemical indicators, iron series indicators and blood routine indicators were not statistically significant (P > 0.05). Conclusion There is no obvious toxicological reaction by nanomagnetic beads. The toxicology related to nanomagnetic beads remain to be further explored.
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[1] 兰天，呼日勒特木尔.脑卒中流行病学现状及遗传学研究进展[J].疑难病杂志，2015，26（9）：986-989.
[2] 刘思维，周立春，贾伟华.脑梗死发病前使用抗栓药物治疗对急性脑梗死后出血转化的影响分析[J].临床和实验医学杂志，2016，15（11）：1039-1042.
[3] 马琴飞，孙灵芬.基质辅助激光解吸电离飞行质谱技术联合纳米磁珠在宫颈癌早期诊断中的应用性研究[J].中国性科学，2014，23（6）：29-32.
[4] 崔敬敬，金光玉，夏雷，等.超顺磁性氧化铁纳米粒在磁共振成像和药物传递系统中的应用[J].国际药学研究杂志，2015，42（5）：601-605.
[5] 邢珍珍，郑济林，陆宵彤，等.超顺磁性氧化铁纳米颗粒作为药物载体的研究进展[J].广东医学，2015，36（22）：3543-3545.
[6] 王荣亮，刘小蒙，张营，等.大鼠局灶性脑缺血后神经功能评分与脑梗死体积相关性分析[J].首都医科大学学报，2013，34（1）：69-74.
[7] 胡镜清，温泽淮，赖世隆.Morris水迷宫检测的记忆属性与方法学初探[J].广州中医药大学学报，2000，17（2）：117-119.
[8] 麦筱莉，范海健，牡丹，等.超顺磁性氧化铁纳米粒子标记内皮祖细胞靶向肝癌的MR实验研究[J].中华核医学与分子影像杂志，2016，36（1）：19-24.
[9] 黄小林，许恒毅.磁性氧化铁纳米粒子的表面修饰及其在CTCs分离中应用的研究进展[J].中国肿瘤临床，2014， 41（1）：56-59.
[10] 陈龙华.磁共振成像观察SPIO标记脂肪干细胞在脑梗死大鼠脑内的迁徙和分布[J].中国组织工程研究，2016， 20（6）：793-798.
[11] 王海龙，张良，郭艾.超顺磁性氧化铁纳米颗粒的理化性质及其标记BMSCs的安全性、示踪时效性研究进展[J].山东医药，2015，55（24）：99-101.
[12] 陈茜.磁性纳米颗粒在医学诊疗中的潜在应用研究[D].济南：山东大学，2016.
[13] 王国斌，肖勇，陶凯雄，等.纳米级四氧化三铁的药物动力学和组织分布研究[J].中南药学，2005，3（1）：5-7.
[14] 孙汉文，张彦聪，宋新峰，等.超顺磁性氧化铁纳米粒子在肿瘤靶向诊断治疗中的应用进展[J].中国材料进展，2016，35（3）：233-236.
[15] 昂盛骏，唐萌.纳米氧化铁的毒性及毒作用机制研究进展[J].毒理学杂志，2015，29（2）：152-157.
[16] 陈柱，尚全良，肖恩华.超顺磁氧化铁制备及在生物医学领域的应用[J].中国组织工程研究，2015，19（43）：7041-7046.
[17] 王雷，胡志远.循环肿瘤细胞纳米检测技术用于肿瘤早期诊断的探索[J].中国肿瘤生物治疗杂志，2016，23（5）：595-600.
[18] 黄长文，蒋星星，殷香保，等.FTC-CD133纳米微粒抑制肝癌干细胞耐药性的研究[J].中国全科医学，2016， 19（2）：184-189.
[19] 李祥，朱文珍，魏黎.氧化铁纳米粒子神经干细胞标记技术的比较研究[J].中国医学影像学杂志，2014，22（1）：7-11.
[20] 史旭东，王晓，申一鸣，等.超顺磁性氧化铁纳米粒子SPION-dopa-PEG-DOTA/RGD的64Cu标记、纯化及生物分布[J].核化学与放射化学，2015，37（4）：250-256. |
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