携载BMP-2的PLGA缓释微球的制备及其成骨活性的研究

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Abstract Objective To research the physical characteristics of novel bone morphogenetic factor-2 (BMP-2)-loaded poly (lactic-co-glycolic acid) (PLGA) microspheres with a sustained control release of BMP-2 for bone induction and the influence ossification in vitro and in vivo. Methods The BMP-2-loaded PLGA microspheres through double emulsion method was prepared, and the physiological properties of microspheres was investigated, including particle size and loading and encapsulation rates of BMP-2, and the release profile of BMP-2. The effects of BMP-2-loaded microspheres on the proliferation and differentiation of bone marrow mesenchymal stem cells (BMSCs) in vitro and the ectopic bone formation inside the mouse thigh muscle were studied. Results The average size of PLGA particles loaded with BMP-2 was (242.2±31.3) μm with smooth surface. BMP-2 released from microspheres displayed a biphasic release profile. BMSCs cultured with BMP-2-loaded microspheres showed the fastest proliferation rate among all groups (P < 0.05). The proliferation rate of BMSCs was dose-dependent on BMP-2 (P < 0.05). Ectopic bone formation in the mouse thigh muscle was observed after injection of BMP-2-loaded-microspheres. A large number of trabecular bone appeared with emerging bone marrow cavity 4 weeks after the injection. Conclusion The new method to prepare PLGA microspheres loaded with BMP-2 has a sustained control release rate, which can promote the proliferation and differentiation of BMSCs in vitro and ectopic bone formation in vivo. BMP-2-loaded PLGA microsphere holds high potential for wide application in bone tissue regeneration.

Key words： PLGA BMP-2 Microspheres Osteogenetic activity Bone tissue

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	WU Yongheng ZHANG Zijun HUANG Yan ZHAO Wen

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WU Yongheng ZHANG Zijun HUANG Yan ZHAO Wen. Study on preparation and osteogenic effects of BMP-2-loaded PLGA microspheres with a sustained control release[J]. 中国医药导报, 2017, 14(23): 25-28.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2017/V14/I23/25

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