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| Analgesic effect of electroacupuncture with different parameters combinationson SNL and endorphin expression of hypothalamus in rats |
| ZHOU Jie1 CHEN Zhenyu2 GONG Jie3 ZHUANG Shengjian4 SHAO Xiaomei5 FANG Jianqiao5▲ |
1.The Third Affiliated Hospital of Zhejiang Chinese Medical University, Zhejiang Province, Hangzhou 310005, China;
2.People's Hospital of Xiacheng District, Zhejiang Province, Hangzhou 310000, China;
3.Traditional Chinese Medical Hospital of Xiaoshan District, Zhejiang Province, Hangzhou 311201, China;
4.Integrated Traditional Chinese and Western Medicine Hospital of Wenzhou, Zhejiang Province, Wenzhou 325000, China;
5.The Third Clinical Medical College of Zhejiang Chinese Medical University, Zhejiang Province, Hangzhou 310053, China |
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Abstract Objective To evaluate the analgesic effect of neuropathic painmodel rat of electroacupuncture with different parameter combinations, select the best parameter combination of electroacupuncture treatment forneuropathic pain. Methods 30 SD male rats were randomly divided into normal group (N group), model group (M group), 2 Hz group，120 Hz group, and 2/120 Hz group, with 6 rats in each group. Neuropathic pain model was established by spinal nerve ligation (SNL). EA groups were given electroacupuncture on ipsilateral of Zusanli (ST36) and Kunlun (BL60). The Paw withdraw thresholds of each rat were measured as an assessment of nociception before and 24 hours after operation and at the 1st, 3rd, 5th, 7th, 9th, 10th day after the treatment. The ENK and β-END concentration of hypothalamus were measured by RIA. Results PWTs of 2 Hz groups was higher than M group in every time point (P < 0.05 or P < 0.01); at the 3rd, 5th, 7th after EA, the PWTs of 2 Hz group were higher than 120 Hz group and 2/120 Hz group (P < 0.05 or P < 0.01); at the 1st, 7th, 9th, 10th after EA, the PWTs of 120 Hz group and 2/100 Hz group were higher than M group (P < 0.05 or P < 0.01). The β-END concentration of hypothalamus of EA groups was higher than M group and N group (P < 0.01). The ENK concentration of hypothalamus of 2/120 Hz EA group was higher than M group, N group, 2 Hz EA group and 120 Hz EA group (P < 0.05 or P < 0.01). Conclusion Different parameter combinations electroacupuncture have analgesic action for neuropathic pain modal rat, but the analgesia effect is different. Electroacupunctrue is effective for neuropathic painrelated to the secretion of endorphins. At early stage of neuropathic pain,2 Hz/50 μs electroacupuncture is advisable, while at late stage of neuropathic pain, different parameter combinations electroacupuncture is similar efficacious.
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