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| Expression of MMRN2 in breast cancer and its influence on prognosis |
| LI Huaping SUN Shengrong▲ CHEN Chuang WANG Yuan |
| Department of Breast and Thyroid Surgery, Renmin Hospital of Wuhan University, Hubei Province, Wuhan 430060, China |
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Abstract Objective To investigate the expression of MMRN2 in breast cancer and its influence on prognosis, discuss the mechanism of MMRN2 in breast cancer. Methods Western blot and qRT-PCR were used to detect the expression of MMRN2 in 50 pairs of human breast invasive ductal carcinoma specimens and adjacent tissues, which had excision by surgery in Renmin Hospital of Wuhan University from January 2013 to April 2017. The relationship between the expression of MMRN2 and the pathologic features and prognosis of patients were analyzed. Results The expression of MMRN2 in breast cancer was down-regulated (P < 0.01), and the expression of MMRN2 was not correlated with the pathological characteristics of patients (P > 0.05). Up-regulated MMRN2 could prolong the overall survival (P = 0.036) and no recurrence survival time (P = 0.019) of patients with breast cancer. Conclusion MMRN2 is a tumor suppressor gene for breast cancer, and can serve as a potential target for molecular treatment of breast cancer, improve patients′ survival.
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[1] Beretov J，Wasinger VC，Graham PH，et al. Proteomics for breast cancer urine biomarkers [J]. Adv Clin Chem，2014， 63：123-167.
[2] Siegel RL，Miller KD，Jemal A. Cancer statistics，2017 [J]. CA Cancer J Clin，2017，67（1）：7-30.
[3] Cancer Genome Atlas Network.Comprehensive molecular portraits of human breast tumours [J]. Nature，2012，490（7418）：61-70.
[4] Germain D. Estrogen carcinogenesis in breast cancer [J]. Edodocrinol Metab Clin North Am，2011，40（3）：473-484.
[5] Yang SN，Li FJ，Chen JM，et al. Kinetic Curve Type Assessment for Classification of Breast Lesions Using Dynamic Contrast-Enhanced MR Imaging [J]. PLoS One，2016， 1（4）：e0152827.
[6] Julia B，Valerie C，Ewan K，et al. Proteomic Analysis of Urine to Identify Breast Cancer Biomarker Candidates Using a Label-Free LC-MS/MS Approach [J]. PLoS One，2015， 10（11）：e0141876.
[7] Lei T，Zhao X，Jin S，et al. Discovery of Potential Bladder Cancer Biomarkers by Comparative Urine Proteomics and Analysis [J]. Clin Genitour Cancer，2013，11（1）：56-62.
[8] Hassanein M，Callison JC，Callaway-Lane C，et al. The state of molecular biomarkers for the early detection of lung cancer [J]. Cancer Prevention Res，2012，5（8）：992-1006.
[9] Beretov J，Wasinger VC，Graham PH，et al. Proteomics for breast cancer urine biomarkers [J]. Adv Clin Chem，2014， 63：123-167.
[10] Rao N，Lee YF，Ge RW. Novel endogenous angiogenesis inhibitors and their therapeutic potential [J]. Acta Pharmacot Sin，2015，36（10）：1177-1190.
[11] Lorenzon E，Colladel R，Andreuzzi E，et al. MULTIMERIN2 impairs tumor angiogenesis and growth by interfering with VEGF-A/VEGFR2 pathway [J]. Oncogene，2012，31（26）：3136-3147.
[12] Welch-Reardon KM，Ehsan SM，Wang K，et al. Angiogenic sprouting is regulated by endothelial cell expression of Slug [J]. J Cell Sci，2014，127（Pt 9）：2017-2018.
[13] Yuan BQ，Xian RH，Ma JF，et al. Isthmin inhibits glioma growth through antiangiogenesis in vivo [J]. J Neuro Oncol，2012，109（2）：245-252.
[14] Van Buul JD，Geerts D，Huveneers S. Rho GAPs and GEFs： controling switches in endothelial cell adhesion [J]. Cell Adh Migr，2014，8（2）：108-124.
[15] Noy PJ，Lodhia P，Khan K，et al. Blocking CLEC14A-MMRN2 binding inhibits sprouting angiogenesis and tumour growth [J]. Oncogene，2015，34（47）：5821-5831.
[16] Blanco R，Gerhardt H. VEGF and Notch in tip and stalk cell selection [J]. Cold Spring Harb Perspect Med，2013， 3（1）：a006569.
[17] Chang GH，Lay AJ，Ting KK，et al. ARHGAP18： an endogenous inhibitor of angiogenesis， limiting tip formation and stabilizing junctions [J]. Small GTPases，2014，5（3）：1-15.
[18] Baker M，Robinson SD，Lechertier T，et al. Use of the mouse aortic ring assay to study angiogenesis [J]. Nat Protoc，2011，7（1）：89-104.
[19] De Angelis JE，Lagendijk AK，Chen H，et al. Tmem2 regulates embryonic vegf Signaling by controlling hyaluronic acid turnover [J]. dev cell，2017，40（2）：123-136.
[20] Andreuzzi E，Colladel R，Pellicani R，et al. The angiostatic molecule multimerin 2 is processed by MMP-9 to allow sprouting angiogenesis [J]. Matr Biol，2017.DOI：10.1016/j.matbio.2017.04.002 |
|
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