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| The study of protective effects of EGCG on oxidative -induced apoptosis in myocardial cells |
| YANG Rui DONG Zhuo WANG Mengqi XU Lei▲ |
| The Third Ward of Cadres, the First Affiliated Hospital of Harbin Medical University, Heilongjiang Province, Harbin 150001, China |
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Abstract Objective To discuss the protective effects and possible mechanisms of epigallocatechin gallate (EGCG) in oxidative damage of rat H9C2 cells induced by oxidative stress. Methods H9C2 cells were random grouped after subcultured. There were negative control group (cultured with normal nutrient solution), H2O2 injury group (incubated with 100 μmol/L H2O2 for 12 h), EGCG low dose group (incubated with 10 μmol/L EGCG for 24 h then cultured with 100 μmol/L H2O2 for 12 h) and EGCG high dose group (incubated with 100 μmol/L EGCG for 24 h then cultured with 100 μmol/L H2O2 for 12 h). Cell viability were tested by MTT colorimetric detection, apoptotic cell morphology was observed by Hochest33258 staining, apoptosis rate was detected by flow cytometry, expression of apoptosis-related factors Caspses-3 and Caspase-9 were tested by colorimetric detection. Results The cell survival rate increased to 66.68% and 78.63% after treated with 10 μmol/L and 100 μmol/L EGCG compared with H2O2 injury group (57.33%), with statistically significant difference (P < 0.05). Hoechst33258 dying and flow cytometry technology results showed that cell apoptosis rate dropped gradually after treated with different concentration of EGCG with H2O2 injury group, with statistically significant difference (P < 0.05). Besides, the expression of Caspase-3 and Caspase-9 were inhibited after treated with EGCG. Conclusion EGCG effective inhibit H9C2 cells oxidative damage by inhibiting the expression of Caspses-3 and Caspase-9, which provide reliable experimental basis for the treatment of injuries in myocardial cells.
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