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| Study on the effects of Trichostatin A on behaviors and expression of brain-derived neurotrophic factor in model rats with depression |
| LI Kun1 SUN Guoping2 LU Haozhong2 SUN Zhongguo2 |
1.Pharmacy Intravenous Admixture Services, Qilu Hospital of Shandong University (Qingdao), Shandong Province, Qingdao 265000, China;
2.Qingdao Mental Health Center, Shandong Province, Qingdao 266034, China |
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Abstract Objective To investigate the effects of Trichostatin A on behaviors and expression of brain-derived neurotrophic factor (BDNF) in model rats with chronic unpredictable mild stress-induced depression (CUMS). Methods Forty adult male SD rats were randomly divided into normal control group, model group, Fluoxetine group and Trichostatin A group. The model of depression was established with alternative unpredictable stress combining isolated living conditions. The rat ethological changes were assessed by body weight, sucrose consumption and open field test. The protein expressions of BDNF and phosphorylated cAMP responsive element binding protein (p-CREB) were measured by Western blot. Results After modeling for 21 days, the body weight, sucrose consumption, horizontal motion and vertical motion scores in model group were significantly lower than those of normal control group (P ＜ 0.05); however, these ethological indexes in Fluoxetine group and Trichostatin A group were obviously increased compared with those of model group (P ＜ 0.05). The results of Western blot showed that the expression of BDNF and p-CREB in model group were significantly lower than those of normal control group (P ＜ 0.05), while the expression of BDNF and p-CREB in Fluoxetine group and Trichostatin A group were significantly higher than those of model group (P ＜ 0.05). Conclusion Trichostatin A can effectively improve depression-like behaviors in CUMS model rats, its anti-depressive effect may be attributed to up-regulation of the CREB-BDNF signaling pathway.
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