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| Change of flash electroretinogram in rats with experimental autoimmune uveitis |
| LIU Zhengfeng1 GUO Dadong2 LI Jiao3 SUN Yuanyuan1 LIU Bin1 BI Hongsheng2,3▲ |
1.Shandong University of Traditional Chinese Medicine, Shandong Province, Ji′nan 250355, China;
2.Eye Institute of Shandong University of Traditional Chinese Medicine, Shandong Province, Ji′nan 250002, China;
3.Affiliated Eye Hospital of Shandong University of Traditional Chinese Medicine, Shandong Province, Ji′nan 250002, China |
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Abstract Objective To observe the changes of flash visual evoked potential (F-VEP) and flash electroretinogram (F-ERG) in experimental autoimmune uveitis (EAU) rats. Methods 12 female Lewis rats (6-8 weeks old) were randomly divided into the control group and the experimental group with a random number table method. Each group included 6 rats. EAU models were induced by injection of interphotor-eceptor retinoid binding protein (IRBP, 1177-1191) supplemented with complete Freund adjuvant (CFA) and tuberculin (TB) at footpads, napes and back of the body; while another 6 rats without any treatment were used as normal controls. After EAU induction for 8 days, both EAU and control rats were examined by Fundus, fundus fluorescein angiography (FFA), optical coherence tomography (OCT), F-VEP and F-ERG techniques, respectively. Results Compared with those in control group, the results of rats in EAU group determined by fundus photograph, OCT and FFA had no apparent alterations after induction for 8 days, and no significant difference in F-VEP measurement was found between the two groups (P ＞ 0.05), however, there was a significant difference for the result of F-ERG between EAU and control groups (P < 0.05). Conclusion The F-ERG in EAU rats has been apparent changed after EAU induction for 8 days, indicating that the retinal cells have been damaged.
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