三氯生在嗜麦芽窄食单胞菌耐药机制中的作用

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Abstract Objective To investigate the changes of Antibiotic resistance of Stenotrophomonas maltophilia and the expression of efflux pump SmeDEF in the induction of Triclosan. Methods From March to June in 2017, 10 strains of Stenotrophomonas maltophilia isolated from bacterial lab cultured in Weifang People′s Hospital were selected. The MIC of Stenotrophomonas maltophilia before and after the induction of Triclosan was compared and the repression level of the mRNA of efflux pump smeD was monitored by RT-PCR. Results The inhibition of 10 strains of bacteria pump sensitive to antibiotics was positive or negative. The levofloxacin MIC of 6 strains was 3-5 dilution higher than that before induction, and the MIC of 6 strains was reduced when the efflux pump inhibitor CCCP was used. The repression level of smeD of both pump inhibitor positive strains antibiotic overnight group and logarithmic growth addition group was significantly higher the no antibiotic group. Conclusion The resistance to antibiotics of Stenotrophomonas maltophilia clinical isolates induced by triclosan has increased, which is related to the expression of efflux pump SmeDEF.

Key words： Tricolsan Stenotrophomonas maltophilia Antibiotic resistance Efflux pump Induction

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	GAO Ruihao1 WANG Yousen1 LIU Demei2▲

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GAO Ruihao1 WANG Yousen1 LIU Demei2▲. The effect of Triclosan on the resistance mechanism of Stenotrophomonas maltophilia[J]. 中国医药导报, 2018, 15(23): 113-116.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2018/V15/I23/113

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