特应性皮炎患者皮肤马拉色菌菌量及其与病情严重程度相关性研究

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Abstract Objective To detect the amount of Malassezia in the skin of patients with atopic dermatitis (AD), and explore its correlation with the disease severity. Methods 20 mild, 20 moderate and 20 severe AD patients were selected from the First Affiliated Hospital of Chengdu Medical College from April to September 2017. Skin scales were collected as samples from the lesion and non lesion areas on the face and upper limb of AD patients as well as the corresponding areas of 20 healthy control subjects. The density of Malassezia was recorded at high magnification by fluorescent staining method. Then the correlation between the density of Malassezia and the disease severity was analyzed. Results The density of Malassezia in the lesion and non lesion areas on the face of the mild, moderate and severe AD patients was significantly higher than those of the upper limbs (P ＜ 0.05). The density of Malassezia in each skin area of mild, moderate and severe AD patients was significantly higher than that of healthy control subjects (P ＜ 0.05). The density of Malassezia in each skin area of AD patients was not correlated with the disease severity (P ＞ 0.05). Conclusion The amount of Malassezia in the skin of AD patients is related to the site, but not to the disease severity.

Key words： Atopic dermatitis Malassezia Fluorescent staining method Disease severity

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	WU Hongmei LU Mao OU Mei LUO Yuanyuan

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WU Hongmei LU Mao OU Mei LUO Yuanyuan. Study on the amount of Malassezia in the skin of patients with atopic dermatitis and its correlation with the disease severity[J]. 中国医药导报, 2018, 15(23): 96-99.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2018/V15/I23/96

[1] Bjerre RD，Bandier J，Skov L，et al. The role of the skin microbiome in atopic dermatitis：a systematic review [J]. Br J Dermatol，2017，177（5）：1272-1278.
[2] Heratizadeh A，Werfel T，Rosner LM. Adaptive immune response and associated trigger factors in atopic dermatitis [J]. Hautarzt，2015，66（2）：96-102.
[3] Jagielski T，Rup E，Ziolkowska A，et al. Distribution of Mal-assezia species on the skin of patients with atopic dermatitis， psoriasis，and healthy volunteers assessed by conventional and molecular identification methods [J]. BMC Dermatol，2014，14（1）：3.
[4] Prohic A，Jovovic ST，Kuskunovic-Vlahovljak S，et al. Distribution of Malassezia Species in Patients with Different Dermatological Disorders and Healthy Individuals [J]. Acta Dermatovenerol Croat，2016，24（4）：274-281.
[5] Leshem YA，Hajar T，Hanifin JM，et al. What the Eczema Area and Severity Index score tells us about the severity of atopic dermatitis： an interpretability study [J]. Br J Dermatol，2015，172（5）：1353-1357.
[6] 赵辨.中国临床皮肤病学[M].2版.南京：江苏凤凰科学技术出版社，2017：772.
[7] Conti Diaz IA，Civila E，Veiga R. The importance of microscopic examination in the management of desquamative diseases of the scalp [J]. Mycopathologia，2002，153（2）：71-75.
[8] 董兴军，张春亭，胡爱萍.糠秕马拉色菌直接镜检的方法学选择[J].皮肤性病诊疗学杂志，2014，21（6）：473-477.
[9] 吴海虹，林家坤，李运华，等.萍乡地区AD流行病学调查及CD4~+CD25~+T细胞在AD发病作用的探讨[J].中国当代医药，2016，23（32）：139-141.
[10] 徐明，张弘，邓劲松，等.马拉色菌显微镜检查的方法学比较[J].福建医药杂志，2011，33（1）：70-72.
[11] 韩德忞，刘原志，朱均昊，等.荧光染色法与KOH湿片法在真菌直接镜检中的应用比较[J].中国真菌学杂志，2016，11（4）：240-242.
[12] 罗云鹏，高仰敏，金云，等.特敏增强荧光染色法在浅部真菌病诊断中的应用[J].临床检验杂志，2017，35（10）：736-738.
[13] 陈锐君，佟韬，李超，等.荧光染色法和KOH湿片法在诊断浅部真菌感染中的效果比较[J].中国真菌学杂志，2016，11（6）：378-380.
[14] Ramos L，Mellado S，Ramadan S，et al. The use of calcofluor white for studying Malassezia species by direct microscopy [J]. Rev Argent Microbiol，2006，38（1）：4-8.
[15] Sugita T，Tajima M，Tsubuku H，et al. Quantitative analysis of cutaneous Malassezia in atopic dermatitis patients using real-time PCR [J]. Microbiol Immunol，2006，50（7）：549-552.
[16] Rerknimitr P，Otsuka A，Nakashima C，et al. The etiopathogenesis of atopic dermatitis： barrier disruption，immunological derangement，and pruritus [J]. Inflamm Regen，2017， 37（1）：14.
[17] Koppes SA，Brans R，Ljubojevic HS，et al. Stratum Corneum Tape Stripping：Monitoring of Inflammatory Mediators in Atopic Dermatitis Patients Using Topical Therapy [J]. Int Arch Allergy Immunol，2016，170（3）：187-193.
[18] Schmid-Grendelmeier P，Ballmer-Weber BK. Atopic dermatitis-current insights into path physiology and management [J]. Ther Umsch，2010，67（4）：175-185.
[19] Eberlein-Konig B，Schafer T，Huss-Marp J，et al. Skin surface pH，stratum corneum hydration，trans-epidermal water loss and skin roughness related to atopic eczema and skin dryness in a population of primary school children [J]. Acta Derm Venereol，2000，80（3）：188-191.
[20] Choi MJ，Maibach HI. Role of ceramides in barrier function of healthy and diseased skin[J]. Am J Clin Dermatol，2005，6（4）：215-223.
[21] Kaga M，Sugita T，Nishikawa A，et al. Molecular analysis of the cutaneous Malassezia microbiota from the skin of patients with atopic dermatitis of different severities [J]. Mycoses，2011，54（4）：e24-e28.
[22] Akaza N，Akamatsu H，Sasaki Y，et al. Cutaneous Malassezia microbiota in atopic dermatitis patients differ by gender and body part [J]. Dermatology，2010，221（3）：253-260.
[23] Glatz M，Bosshard PP，Hoetzenecker W，et al. The Role of Malassezia spp. in Atopic Dermatitis [J]. J Clin Med，2015，4（6）：1217-1228.
[24] Glatz M，Buchner M，Von BW，et al. Malassezia spp.-specific immunoglobulin E level is a marker for severity of atopic dermatitis in adults [J]. Acta Derm Venereol，2015， 95（2）：191-196.
[25] Holleran WM，Takagi Y，Uchida Y. Epidermal sphingolipids：metabolism， function， and roles in skin disorders [J]. FEBS Lett，2006，580（23）：5456-5466.
[26] 余昊，张伟.特应性皮炎患者血清总IgE、过敏原特异性IgE、嗜酸性粒细胞检测的临床意义[J].中国医药科学，2018，8（1）：136-138，148.
[27] 余剑兰，李志坚，王佳.伊曲康唑联合制霉菌素片治疗复发性糠秕孢子毛囊炎疗效观察[J].中国医药科学，2018，8（7）：93-95，162.