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| Diagnostic evaluation of three methods for detecting Helicobacter pylori in clinical digestive tract related diseases |
| JIAO Bingxin* GUO Jie* XU Xinmin HE Yanqun ZHOU Chun LI Ping ZHANG Guifang |
| Department of Clinical Laboratory, Beijing Ditan Hospital, Capital Medical University, Beijing 100013, China |
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Abstract Objective To evaluate three diagnostic methods for detecting Helicobacter pylori (Hp) infection in clinical digestive tract related diseases. Methods A total of 177 cases treated in Beijing Ditan Hospital from January 2017 to December 2017 were collected, the Hp was detected via C-urea breath test (13C-UBT), rapid colloidal gold and Western blot methods. 13C-UBT method was used as golden standard and rapid colloidal gold and Western blot methods were compared in the sensitivity and specificity. Results Of the 177 cases, 106 cases were positive in 13C-UBT test, 71 cases were negative. Among 106 cases of 13C-UBT positive patients, 100 cases were positive in colloidal gold assay method and 6 cases were negative. The sensitivity rate was 94.3% and the specificity rate was 83.1%; the positive predictive value was 89.3% and the negative predictive value was 90.8%. Among 106 cases of 13C-UBT positive patients, 102 cases were positive and 4 cases were negative by the method of Western blot method. The sensitivity rate was 96.2%, specificity rate was 84.5%; the positive predictive value was 90.3% and the negative predictive value was 93.8%. There was no significant difference between the two methods (P ＞ 0.05). There were 147 cases of different gastrointestinal diseases were detected by the method of Western blot, of which 66 cases were type Ⅰ, accounting for 44.9%; type Ⅱ 20 cases, accounting for 13.61%. There was a significant difference between the two subtypes (χ2 = 34.778, P = 0.000). The subtypes distribution of Hp, type Ⅰ and Ⅱ had a significant difference between gastroduodenal ulcer and chronic gastritis (P ＜ 0.05), while the distribution in reflux esophagitis had no difference (P ＞ 0.05). Conclusion The rapid colloidal gold method is highly correlated with the infection of Hp and is suitable for the preliminary screening of Hp infection. However, the method of Western blot can be used for toxicity typing and is more suitable for the choice of clinical drug treatment.
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