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| Pharmacological activity of Qufeng Jiejing Decoction in treating cough variant asthma |
| SONG Ping ZHANG Xi ZHANG Tong ZHANG Zhongde |
| Lingnan Zhen Shi Miscellaneous Disease School Outpatient, Guangdong Province Traditional Chinese Medical Hospital, Guangdong Province, Guangzhou 510120, China |
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Abstract Objective To preliminarily investigate the efficacy of Qufeng Jiejing Decoction on airway inflammation reaction in guinea pigs with cough variant asthma (CVA). Methods Forty SPF female guinea pigs were divided into five groups with eight Digs in each group by random number table, including control group, model group, positive drug group, low-dose Qufeng Jiejing Decoction group and high-dose Qufeng Jiejing Decoction group. The OVA-induced method, by injecting OVA and aluminum hydroxide (ALP) intraperitoneally, was applied to make guinea pig CVA model. On the date of nebulization, the positive drug group, high-dose and low-dose Qufeng Jiejing Decoction groups were respectively treated with prednisone acetate at 3 mg/kg, Qufeng Jiejing Decoction at doses of 2.88 g/kg and 8.22 g/kg by gavage. The control group and model group were treated with normal saline at the same time. The cough response sensitivity (CRS), eosinophil (EOS) percentage in bronchoalveolar lavage fluid (BLAF), as well as levels of serum interleukin -4 (IL-4), IL-12, IL-13, tumor necrosis factor β (TNF- β) and interferon-γ (IFN-γ) of each group were detected. Results Compared with the control group, the CRS, EOS percentage, and the expression levels of IL-4, IL-12, IL-13, TNF-β and INF-γ in serum of the model group were increased significantly (P ＜ 0.05). Compared with the model group, the CRS and EOS percentage were significantly decreased in the positive drug group, high-dose and low-dose Qufeng Jiejing Decoction group, while the levels of serum IL-4, IL-12, IL-13, TNF-β and INF-γ were significantly reduced (P ＜ 0.05). Compared with the low-dose Qufeng Jiejing Decoction group, the CRS, EOS percentage, and the levels of TNF-β and INF-γ in high-dose Qufeng Jiejing Decoction group were reduced significantly (P ＜ 0.05). Conclusion Qufeng Jiejing Decoction can evidently inhibit the CRS and the EOS percentage of CVA guinea pigs, and also reduce the expression of inflammatory cytokines in their serum to some extent. However, the relevant mechanism is worth investigating and needs further exploration.
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