经小脑延髓移植骨髓间充质干细胞对受损脊髓白质的影响

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Abstract Objective To investigate the effect of bone marrow stem cells (BMSCs) transplanted via cisterna magna on the rehabilitation of damaged spinal cord white matter. Methods The spinal cord injury (SCI) model was carried out by a modified Allen method, assigned into the control group and BMSCs group randomly. The BMSCs group and control group were given 10 μL BMSCs suspension and 10 μL physiological saline via cistern magna respectively. BBB scale was utilized to estimate the neurological recovery on 1, 7, 14, 21, 28 d postoperatively after SCI. The white matter changes in the injured spinal cord were tested using 4.7 T MRI scanner. HE staining was applied to access the rehabilitation condition. Flourescence microscope was performed to identify the signal level of Hoechst 33342 marked BMSCs in the injured spinal cord. Results The BBB scores in the BMSCs group were significantly higher than that of the control group on 7, 14, 21, 28 d after SCI (P < 0.05). There was no statistically significant difference between the two groups regard to the longitudinal diffusion gradients of the damaged spinal cord white matter (P > 0.05). The average size of the lesion center in the BMSCs group was significantly smaller than that of the control group (P < 0.05). HE staining revealed that the recovery of the BMSCs group was better than the control group at 14, 28 d after SCI. The counts of Hoechst 33342 labelled cells were less in the control group than that in the BMSCs group at 7 d. Subsequently, they continued to decrease from 7 d to 4 weeks later, with highly statistically significant difference (P < 0.01). Conclusion Bone marrow mesenchymal stem cells transplanted via cisterna magna has a positive role in promoting the rehabilitation of damaged spinal cord white matter, which is a novel way of treating SCI.

Key words： Bone marrow stem cells Cisterna magna Spinal cord injury Demyelinating changes

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	MING Chao ZENG Yun XIONG Min

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MING Chao ZENG Yun XIONG Min. Effects of bone marrow stem cells transplanted via cisterna magna on injured spinal cord white matter[J]. 中国医药导报, 2018, 15(22): 9-13.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2018/V15/I22/9

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