丹参注射液对大鼠下颌骨骨折愈合过程中BMP-2和TGF-β1的影响

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Abstract Objective To investigate the effect of Danshen Injection on mandibular fracture of healing in rat and observe the changes of bone morphogenetic protein-2(BMP-2), transforming growth factor-β1(TGF-β1). Methods Forty-eight SD rats were operated for incomplete fracture of hibateral mandible penetrated from buccal side to lingual side. The rats were divided into experimental group and control group according to random number table, with 24 rats in each group. The experiment group was administered with Danshen Injection by intraperitoneal injection, and the control group was administered with physiologic saline by intraperitoneal injection. The right healing of fracture were used to detect fracture healing by X-ray and to measure the average bone density of the callus area, the percent trabecular and trabecular width were analyzed by bone histomorphometry and the serum levels of BMP-2, TGF-β1 was determined by ELISA at 1, 2, 3, 4 weeks after the operation. The right healing of fracture were comminuted, and the expression of BMP-2 and TGF-β1 were analyzed by RT-qPCR. Results The X-ray results showed that the fracture healing in the control group was obviously delayed in the experimental group. After 2 weeks, compared with the control group, the callus bone mineral density (BMD) in the experimental group was increased (P < 0.05 or P < 0.01). The trabecular area ratio and trabecular width in the callus area were markedly increased (P < 0.05 or P < 0.01), and the difference was even more increased along with the increasing time. ELISA results showed the serum levels of BMP-2 and TGF-β1 in the experimental group were higher than those in the control group (P ＜ 0.05). RT-qPCR results showed the expression of BMP-2 and TGF-β1 in the experimental group were higher than those in the control group from the 2nd week after operation (P ＜ 0.01). Conclusion Danshen Injection can promote the healing of mandibular fracture, which may be related to the transcription and protein expression of BMP-2 and TGF-β1 gene during the process of fracture healing.

Key words： Danshen Injection Mandibular fracture Bone morphogenetic protein-2 Transforming growth factor-β1

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XIANG Le YU Wei. Effect of Danshen Injection on expression of BMP-2 and TGF-β1 during healing of rat mandibular fracture[J]. 中国医药导报, 2018, 15(22): 4-8.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2018/V15/I22/4

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