|
|
|
| Effects of flavonoids extract from Pteris multifida Poir on photoaged skin of mice |
| CHEN Jiaming ZHANG Meiqin CHEN Ning LUO Xu YANG Suming CHEN Yongbei XIE Chengwen |
| The First Clinical College, Changsha Medical University, Hu'nan Province, Changsha 410219, China |
|
|
|
Abstract Objective To investigate the specific repairing effects of flavonoids extract from Pteris multifida Poir on photoaged skin of mice caused by ultraviolet irradiation, and to provide a theoretical basis for the medicinal use of this plant. Methods Forty damaged model mice with photoaging caused by ultraviolet irradiation were selected, according to the gender, they were divided into group A (no treatment), group B (smeared normal saline), group C (smeared normal saline + vitamin C), group D (smeared normal saline + flavonoids rutin extract), with 10 mice in each group. Follow-up observation of 10 days, the differences of surface evaluation of roughness (SER), surface evaluation of scales (SESC), surface evaluation of wrinkles (SEW), percentage of healed area, microvessel density (MVD), skin healing time, scab time and fluff generated time among the four groups were compared. Results In the 7th and 10th days, the SER, SESC, and SEW in group A, B, and C were all significantly higher than those of group D (all P ＜ 0.05), the percentage of healed area and MVD were significantly lower than those of group D (all P ＜ 0.05). The skin healing time, scab time and fluff generated time in group A, B, C were significantly shorter than those of group D (all P ＜ 0.05), and the results of each index in group A were maximum, and the results of each index in group D were minimum. Skin histopathologic examination showed that, the skin collagen fibre in group A showed no regular fracture, the emptiness of the dermis in group B was enlarged gradually, the epidermis of group C was relatively intact, the epidermis of group D had better recovery conditions and collagen fibers were also more than other groups. Conclusion Biological flavonoids rutin substances contribute to the repair of photoaged skin, reflected in the skin appearance and internal organizational structure, the efficacy is better than vitamin C, which can greatly enhance the skin healing time.
|
|
|
|
|
|
[1] 王甜甜.江南卷柏提取物的纯化分析及其对皮肤伤口愈合作用的研究[D].济南：山东大学，2016.
[2] 贺栋业，李晓宇，王丽丽，等.金花茶化学成分及药理作用研究进展[J].中国实验方剂学杂志，2016，22（3）：231-234.
[3] Pedrol JG，Trull JL，Sánchez MC，et al. Clinical observation of a professional tattooing procedure and evolutionary study of the skin damage provoked [J]. Photonics Lasers Med，2016，5（1）：23.
[4] 刘大旭，高晓波，祁永华，等.杜仲有效成分β-萘黄酮对UVB诱导人皮肤成纤维细胞光老化保护作用研究[J].中医药学报，2016，44（3）：41-43.
[5] 路婷婷，陈亚泽，卢涛，等.紫外线的皮肤损伤机制及具有紫外线防护作用的天然产物的研究进展[J].中国药理学通报，2012，28（12）：1655-1659.
[6] 周国梁，周贤，刘汉珍，等.凤尾草总黄酮超声波提取工艺研究[J].安徽科技学院学报，2012，26（3）：39-44.
[7] Zhao Y，Zhao K，Jiang K，et al. A Review of Flavonoids from Cassia Species and their Biological Activity [J]. Curr Pharm Biotechnol，2016，17（13）：1134-1146.
[8] 沈鹏.林蛙皮多肽对兔皮肤损伤的修复作用研究[D].吉林：吉林大学，2016.
[9] 陈慧.欧亚旋覆花总黄酮对D-半乳糖诱导小鼠衰老模型的抗皮肤衰老作用及其机制的研究[D].重庆：重庆医科大学，2016.
[10] 葛玉珍.芦荟凝胶对激光所致皮肤损伤的修复效果分析[J].中国现代药物应用，2016，10（22）：137-138.
[11] Dennerlein K，Kiesewetter F，Kilo S，et al. Dermal absorption and skin damage following hydrofluoric acid exposure in an ex vivo human skin model [J]. Toxicol Lett，2016，248：25-33.
[12] 杨笛，蒋献.川陈皮素治疗皮肤疾病的研究进展[J].华西药学杂志，2016，31（1）：107-108.
[13] 刘欣.紫外线治疗仪在肠道移植物抗宿主病所致的肛周皮肤损伤中的应用及临床分析[J].中国妇幼健康研究，2016，27（2）：248.
[14] 涂云华，陈利远，王喜，等.芳姜黄酮衍生物对WM35细胞增殖及凋亡的影响[J].山东大学学报：医学版，2017， 55（2）：68-73.
[15] 吕燕红.枸杞叶总黄酮对UVB照射致无毛小鼠皮肤光损伤的防护作用研究[D].兰州：兰州大学，2016.
[16] 张勰.野菊花CO2超临界萃取物对D-半乳糖及UV致衰老小鼠的保护作用[D].广州：广州中医药大学，2016.
[17] 付维维，刘娟，王业秋，等.甘草黄酮对UVB损伤HDF细胞保护作用研究[J].安徽农业科学，2016，44（4）：170-171，174.
[18] 肖辉.甘草黄酮对UVB诱导HaCaT细胞ERS中IRE1信号通路的影响[D].兰州：兰州大学，2016.
[19] 吴海游，邱楚群，梁美婷，等.人参辅酶Q10防晒霜对紫外线损伤小鼠的保护作用[J].中国实验动物学报，2016， 24（6）：601-606. |
|
|
|
