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| Effects of formononetin on proliferation and apoptosis of human bladder cancer cells T739 and its mechanism |
| LIU Qing1 LIANG Meihua2 ZHANG Xing2 LI Zhengzhao3 CAO Jun1 |
1.Department of Oncology, the First People′s Hospital of Qujing, Yunnan Province, Qujing 655000, China;
2.Laboratory of Tumor Immune and Micro Environmental Regulation, Guilin Medical University, Guangxi Zhuang Autonomous Region, Guilin 541004, China; 3.Department of Emergency, West Brance, the First Affiliated Hospital of Guangxi Medical University, Guangxi Zhuang Autonomous Region, Nanning 530007, China |
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Abstract Objective To study the effect of formononetin on the proliferation and apoptosis of human bladder cancer cells T739 cells, and discuss the mechanism. Methods Different contents of 0, 15, 30, 60 μmol/L formononetin were added to T739 cells. CCK8 assay was applied to determine the effect of formononetin on proliferation of T739 cells. Apoptosis was analyzed by flow cytometry and Hoechst33258 staining. The expressions of GSK-3β, Axin and β-catenin proteins in T739 cells were determined by Western Blot. Results Different contents of 15, 30, 60 μmol/L formononetin treatments suppressed the proliferation of T739 cells in a time- or dose-dependent manner (P < 0.05), and the apoptotic rates of formononetin-treated T739 cells for 48 hours were increased in a dose-dependent manner (P < 0.05). In addition, Hoechst 33258 staining revealed that the apoptotic cells of formononetin-treated T739 were increased in a dose-dependent manner. Further, Western blot results showed that formononetin significantly up-regulated the protein levels of GSK-3β and Axin in a dose-dependent manner (P < 0.05), while the protein level of β-catenin was inhibited (P < 0.05). Conclusion Formononetin exerts inhibitory growth effect and induced apoptosis on human bladder cancer of T739 cells. The underlyng mechanism involved may be associated with activating GSK-3β and Axin expressions and inhibiting β-catenin activity in T739 cells.
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