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| The effect of 15-LOX-1 on proliferation of pulmonary arterial endothelial cells and inflammatory cytokines by LPS |
| LIU Ye XING Wenjing LI Sijia |
| Department of Immunology, Harbin Medical University, Heilongjiang Province, Harbin 150081, China |
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Abstract Objective To explore the effect of 15-LOX-1 on proliferation of pulmonary arterial endothelial cells (PAECs) and inflammatory cytokines by LPS. Methods PAECs were cultured in vitro, and divided into 4 groups, including control group, LPS group, 15-LOX-1 siRNA scramble group (scramble group) and 15-LOX-1 siRNA group. The expression of 15-LOX-1 mRNA was detected by Real-time PCR. The proliferation of PAECs was examined by MTT. The expressions of 13-HODE, IL-1α and IL-6 were detected by ELISA. Results In the 15-LOX-1 siRNA group, the expression of 15-LOX-1 mRNA was reduced, the proliferation of PAECs was increased, and the secretions of 13-HODE, IL-1α and IL-6 were reduced compared with the LPS group with statistically significant difference (P < 0.05)Conclusion Down-regulation of 15-LOX-1 can inhibit inflammatory cytokines, regulate PAECs proliferation, and improve endothelial injury induced by LPS.
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