连接SelS基因的携P-选择素单抗靶向微泡结合超声治疗早期糖尿病肾病的实验研究

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Abstract Objective To study the effect of monoclonal antibody targeted microbubble carrying P-selectin and connecting SelS gene in combination with ultrasound in treating early diabetic nephropathy. Methods A total of 60 specific pathogen free SD male rats were selected as the research objects to build type 2 diabetes rat models. Then the rat models were randomly divided into the control group, SelS group, SMBp group and UE+SMBp group, each group had 15 rats. The food intake, water intake, urine volume, body weight, fasting blood-glucose, 24 h urine protein, β2 microglobulin, blood and urine biochemical indexes, P-selectin, Ang Ⅱ, TGF-β1 and SelS levels of rats in the four groups were compared. Results Before treatment, the differences in general conditions of rats among the four groups were not statistically significant (P > 0.05). The differences in fasting blood-glucose, 24 h urine protein, β2 microglobulin level, blood and urine biochemical indexes, P-selectin, Ang Ⅱ, TGF-β1 and SelS levels were statistically significant after treatment (P < 0.05). Conclusion Monoclonal antibody targeted microbubble carrying P-selectin and connecting SelS gene in combination with ultrasound in treating early diabetic nephropathy has definite therapeutic effect and high safety, thus it can be used as an effective means for prevention and treatment of early diabetic nephropathy.

Key words： P-selectin Monoclonal antibody targeted microbubble Diabetic nephropathy

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	WANG Yan1 LI Jing2▲ HUANG Ruizhu3 HUANG Ping1 HUANG Yuyu2

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WANG Yan1 LI Jing2▲ HUANG Ruizhu3 HUANG Ping1 HUANG Yuyu2. Experimental study of monoclonal antibody targeted microbubble carrying P-selectin and connecting SelS gene in combination with ultrasound in treating early diabetic nephropathy[J]. 中国医药导报, 2017, 14(31): 15-18,23.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2017/V14/I31/15

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