谷胱甘肽肌肤凝胶剂对大鼠皮肤浅Ⅱ度烫伤创面愈合的实验研究

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Abstract Objective To observe the effects of glutathione skin gel on wound healing of superficial second-degree burn in rats. Methods 60 SPF male SD rats were divided into normal group, model group, growth factor group, Kangfuxin group, low-dose and high-dose glutathione skin gel groups, each group had 10 rats. The superficial second-degree burn model was successfully established by scalding the hair-removed area (3 cm × 3 cm) of rats with 75℃ water bath for 25 s. The rats were subjected to application of glutathione skin gel on wound without coverage (1 mL, bid) for 16 days, and recombinant human basic fibroblast growth factor and Kangfuxin were used as control. The wound healing process and whole body conditions of animals (physical signs and scabs formation) were observed daily for 20 days. Results Compared with the model group, treatment of high-dose glutathione skin gel shortened the time of wound healing and getting rid of scabs, reduced healing area in superficial second-degree burn model of rats (P < 0.05 and P < 0.01). Histopathological examination results showed that the wound scab was visible in model group, but with excessive inflammatory exudate. Granulation tissue had been repaired and regenerated under the scabs, and small quantities of epithelia had been regenerated around the wound edge, covering the wound gradually. Compared with the model group, all other groups promoted wound healing and decreased inflammatory exudates. And regeneration of the granulation tissue and epithelia were more obvious and significant in these groups. High-dose glutathione skin gel group and growth factor group had comparable effects on the wound healing. Conclusion Glutathione skin gel significantly promotes wound healing of superficial second-degree burn in rats.

Key words： Glutathione skin gel Superficial second-degree burn model Pathological histology Wound scab Wound healing

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	WANG Qing1 TAO Jishan2 ZHU Xuanxuan1 ZHANG Di1 YANG Chen1 FU Rui1

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WANG Qing1 TAO Jishan2 ZHU Xuanxuan1 ZHANG Di1 YANG Chen1 FU Rui1. Experiment study of healing effects of glutathione skin gel on superficial second-degree burn wounds in rats[J]. 中国医药导报, 2017, 14(31): 9-14.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2017/V14/I31/9

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