维生素E对大鼠心肌缺血-再灌注损伤的影响及机制探讨

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Abstract Objective To discuss the influence and mechanism of vitamin E (Vit E) for myocardial ischemia-reperfusion injury (MIRI) of rats. Methods Sixty male health Wistar rats were selected and divided to Vit E group, model group and control group by random number table method, with 20 rats in each group. For the first two groups, the left anterior descending (LAD) in 2 mm near inferior margin of left auricle was ligatured, the knot was released after 30 min and taken reperfusion, then MIRI model was duplicated. For the control group, the silk yarn just was threaded through LAD rather than taken reperfusion. For Vit E group, 4.5 mg/(kg·d) of Vit E injection was injected intramuscularly as soon as the molding was done; for the model group and control group, 4.5 mg/(kg·d) of normal saline was injected intramuscularly after the operation, and the administration was conducted successively for 2 weeks. The influences of different intervention on the antioxidant index, degree of ischemic infarction and hemodynamic index in each group were tested and compared. Results ①Compared with model group, the left ventricular end systolic pressure (LVESP) in Vit E group was significantly increased, while the left ventricular end-diastolic pressure (LVEDP), atrial natriuretic peptide (ANP) were significantly decreased, the differences were highly statistically significant (P ＜ 0.01); ②compared with the model group, the contents of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), nitric oxide (NO) activity in Vit E group were significantly increased, while the contents of malondialdehyde (MDA) were significantly decreased, the differences were highly statistically significant (P ＜ 0.01); ③compared with model group, the area of infarction area (IA) and ischemia risk area (IDA), IA/IDA, IDA/LV in Vit E group were significantly decreased, the differences were highly statistically significant (P ＜ 0.01). Conclusion Vit E can improve the MIRI of rats and enhance the cardiac function through antioxidant stress, oxygen free radical removal effects.

Key words： Myocardial ischemia-reperfusion injury Oxidative stress Vitamin E Hemodynamics

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	CHEN Lingling1* FANG Kan1* LI Longti2 WANG Ping1 PENG Guihai1 WANG Chongquan1

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CHEN Lingling1* FANG Kan1* LI Longti2 WANG Ping1 PENG Guihai1 WANG Chongquan1. Discussion on the influence and mechanism of vitamin E for myocardial ischemia-reperfusion injury of rats[J]. 中国医药导报, 2018, 15(20): 12-15.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2018/V15/I20/12

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