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| Expression of brain-specific angiogenesis inhibitor 1 in colorectal cancer and its correlations with clinicopathological features of colorectal cancer patients |
| WEI Jianchang* HU He* WANG Qiang CHEN Zhuanpeng YANG Ping ZHANG Tong CAO Jie |
| Digestive Disease Center, the First People′s Hospital of Guangzhou City, Guangzhou Medical University, Guangdong Province, Guangzhou 510180, China |
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Abstract Objective To explore the roles of brain-specific angiogenesis inhibitor 1 in colorectal cancer and its correlations with clinicopathological features and prognosis of CRC patients. Methods Fom May 2016 to July 2017, tissue microarray with 208 CRC, 8 normal colon tissues and the clinical information was purchased. The cancer genome atlas (TCGA) dataset is a publicly available dataset with 192 primary CRC patients and the mRNA expressions. BAI1 in TMA of CRC and benign glandular epithelium cells was checked by immunohistochemistry. The exlpore the connection between the express of brain-specific angiogenesis inhibitor 1 with clinicopathological features. Overall survival curve was analyzed by Kaplan-Meier method and Log-rank test. Univariate analysis and multivariate analyses were analyzed with Cox proportional hazards regression to assess the prognostic value of high BAI1 expression for CRC patients. Results BAI1 was localized in the cytoplasm of CRC and benign glandular epithelium cells, showing higher protein levels in CRC tissues compared with normal colon samples (P < 0.001). High BAI1 protein expression was associated with high pathological grade (P = 0.039), advanced clinical stage (P = 0.004) and enhanced tumor invasion (P = 0.006). The cancer genome atlas (TCGA) mRNA expression further showed that BAI1 was upregulated in CRC with advanced clinical stage (P = 0.027) and enhanced tumor invasion (P = 0.021). Kaplan-Meier survival curves revealed that CRC patients with higher BAI1 mRNA levels had shorter overall survival than those with lower expression (P = 0.02). High BAI1 mRNA expression was an independent influence of factor for prognosis of CRC (HR = 2.895; 95%CI: 1.012-8.281; P = 0.047). Conclusion BAI1 ralates to aggressive CRC. High BAI1 expression may predict poor prognosis of CRC patients.
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