鸦胆子苦醇对非小细胞肺癌Nrf2-Notch1信号轴的影响机制研究

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Abstract Objective To explore the effects of Brusatol in the proliferation of A549 and H460 cells of non-small cell lung cancer. Methods The median inhibitory concentration (IC50) of Brusatol for A549 and H460 cells was assessed by methyl thiazolyl tetrazolium (MTT) assay. Through hoechst33258 staining, the morphological changes of cell apoptosis were observed under the inverted fluorescence microscope. The cell viability was detected by CCK-8. The cell apoptosis was tested by Annexin-V-FITC/PI staining. The expression of Nrf2 and Notch1 protein of A549 and H460 cells was measured by Western blot. Results The IC50 of A549 and H460 cells dealt with Brusatol was (0.70±0.03), (0.47±0.05) μmol/L respectively. The results of flow cytometry showed that the apoptosis rates of A549 and H460 cells dealt with Brusatol were increased obviously, which had highly statistically significant differences compared with those of control group (all P ＜ 0.01). The results of Western blot showed that Brusatol could decrease the expression of Nrf2 and Notch1 protein. Conclusion Brusatol can inhibit the growth of A594 and H460 cells, the mechanism may be related to the inhibition of Nrf2-Notch1 axis.

Key words： Brusatol Non-small cell lung cancer Nrf2 Notch1 Cell growth

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	WANG Min LIU Qili XU Shuai LI Pinyu LI Juntao▲

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WANG Min LIU Qili XU Shuai LI Pinyu LI Juntao▲. Study on the mechanism of the influence of Brusatol for the Nrf2-Notch1 axis of non-small cell lung cancer[J]. 中国医药导报, 2018, 15(19): 16-19.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2018/V15/I19/16

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