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| Effect and mechanism of overexpression of ATF4 on the apoptosis of HepG2 cells |
| XIAO Tingyan1 DUAN Wuxia1 XU Ziwei1 ZHOU Shouhong2 ZENG Bin1 |
1.Department of Gastroenterology, the First Affiliated Hospital of University of South China, Hu'nan Province, Hengyang 421001, China;
2.Teaching and Research Office of Physiology, Medical College, University of South China, Hu'nan Province, Hengyang 421001, China |
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Abstract Objective To observe the effect of overexpression of activating transcription factor 4 (ATF4) on the apoptosis of HepG2 cells and explore the mechanism. Methods ATF4 expression plasmid was built. Hepatocellular carcinoma line HepG2 cells were transfected with ATF4 expression plasmid through liposome. The plasmids were divided into blank control group, empty plasmid group and ATF4 plasmid group. The apoptosis rate was detected by flow cytometry. The content of lactate in cell culture medium was tested. The expression of Warburg effect protein pyruvate kinase subtype M2 (PKM2) was measured by Western blot. Results Compared with the empty plasmid group, the apoptosis rate of HepG2 cells increased significantly, the content of lactate in cell culture medium decreased significantly and the expression of PKM2 decreased significantly (all P ＜ 0.05). Conclusion Overexpression of ATF4 promotes the apoptosis of hepatocellular carcinoma line HepG2 cells, the mechanism may be related to inhibition of Warburg effect.
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