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| Structure and function of the CRISPR/cas9 gene editing system and its application progress in glioma |
| WANG Chaojia WANG Hui |
| The Third Department of Neurosurgery, Taihe Hospital Affiliated to Hubei Medical College, Hubei Province, Shiyan 442000, China |
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Abstract Glioma is the most common primary malignant tumor of the brain. Currently, surgery, chemotherapy and radiotherapy are not effective. Gene therapy may become an important development direction of glioma in the future. The existing gene editing techniques have RNA interference, TALEN, ZENs and so on. Because of their complex design operation and high miss distance, they have not been used well. (CRISPR) /CRISPR-associated (Cas) 9 is a natural immune system for many bacteria and most of the palaeophytes. By specific identification of the invaded virus and nucleic acid, the Cas protein is used to cut the virus to achieve its own immunity. Compared with the above methods, it has obvious advantages. This article reviews the structure and function of CRISPR/Cas9 and its application in the treatment of glioma, in order to provide a new therapeutic approach for the treatment of brain glia.
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