|
|
|
| Mechanism of Danhong Decoction in protecting cerebral ischemia reperfusion injury |
| ZHENG Jun1 CHEN Shaoxiu2 ZHANG Xiaochun1 |
| 1.Faculty of Pharmaceutical Sciences, Suizhou Central Hospital Suizhou Hospital Affiliated to Hubei Medical College, Hubei Province, Shuizhou 441300, China; 2.Department of Cardiovascular Medicine, Taihe Hospital Taihe Hospital Affiliated to Hubei Medical College, Hubei Province, Shiyan 442000, China |
|
|
|
Abstract Objective To study the protective mechanism of Danhong Decoction on cerebral ischemia-reperfusion injury. Methods The 48 SD rats were divided into MCAO group, positive drug group (Nebivolol) and observation group (Danhong Decoction) by random number table, with 16 cases in each group, which were in accordance with the modified focal cerebral ischemia reperfusion model (MCAO). They were intervened for one week. Before and after intervention, Longa neurological scores were taken to measure cerebral infarct volume and NO, nitric oxide synthase (eNOs), superoxide dismutase (SOD) and malondialdehyde (MDA) in cerebral infarct tissue and Ca2+ content were detected. The endothelin-B (ET-B) receptors in cerebral cortical microglia and the expression of vascular endothelial growth factor (VEGF) protein and its gene in infarcted brain tissue were compared. Results After intervention, the levels of NO, eNOs, SOD, VEGF-mRNA, VEGF protein and Longa neurological score in the observation group were increased, while the levels of MDA, ET-B protein, ET-B mRNA, Ca2+ and infarct volume were decreased, with statistically significant differences (P < 0.05). After intervention, the differences of those indices between the observation group and the MCAO group were statistically significant (P < 0.05). Besides, the differences of VEGF-mRNA, VEGF protein, MDA and SOD levels between the observation group and the positive drug group were statistically significant (P < 0.05). Conclusion Danhong Decoction has a protective effect on cerebral ischemia reperfusion injury.
|
|
|
|
|
|
[1] 邓珊，潘丽雅，阳洪，等.急性脑梗死患者静脉溶栓前进行阿托伐他汀预处理的疗效及安全性分析[J].中国医药导报，2017，14（19）：55-58.
[2] 王妍，许能贵，曹月红，等.丹红注射液联合依达拉奉治疗急性脑梗死有效性及安全性系统评价[J].医药导报，2016，35（7）：780-788.
[3] 李龙珠，刘家军，陈德森，等.丹红注射液对大鼠局灶性脑缺血再灌注损伤血管内皮生长因子表达的影响[J].实用药物与临床，2015（8）：897-900.
[4] 高来顺.丹红注射液治疗急性脑梗死的临床疗效[J].中国实用神经疾病杂杂志，2017，20（3）：95-97.
[5] 孙念霞，高维娟，易忠良，等.改良线栓法制作SD大鼠局灶性脑缺血再灌注模型[J].中国组织工程研究，2014， 18（2）：225-230.
[6] Longa EZ，Weinstein PR，Carlson S，et al. Reversible middle cerebral artery occlusion without craniectomy in rats [J].Stroke，1989，20（1）：84-91.
[7] 肖沉，孙莉，曹杉杉，等.尾静脉注射不同剂量羟基红花黄色素A的缺血再灌注大鼠脑组织3-NT、iNOS、NO表达观察[J].山东医药，2017，57（11）：15-18.
[8] 张雪，王琦，刘丽君，等.大鼠脑创伤后MKP-1表达上调、NO的生成减少[J].基础医学与临床，2015，11（8）：22-27.
[9] 卢丽敏，段亚莉，林杰武，等.血清MMP-9、cystatin c、NO水平与脑梗死后认知功能障碍关系的研究[J].临床和实验医学杂志，2017，16（5）：452-454.
[10] 南毛球，戴军，黄光辉，等.瑞舒伐他汀治疗血管性痴呆的效果及对血管内皮功能的影响[J].中国医药导报，2016， 13（33）：137-140.
[11] 戚柳杰，裘益仁.丹红注射液联合氯吡格雷对急性缺血性脑卒中患者血浆大内皮素1、D-二聚体及血清脂蛋白（a）的影响[J].浙江中医杂志，2016，51（6）：464-464.
[12] 邓远琼，刘伯胜，邓远琪，等.急性脑梗死患者血管内皮功能和同型半胱氨酸水平变化及其治疗[J].中国全科医学，2013，16（26）：3057-3061.
[13] 刘超，刘敬霞，黑常春.回医扎里奴思方对痰热腑实证脑缺血再灌注大鼠肿瘤坏死因子-α和内皮素-1表达的影响[J].中国老年学杂志，2017，37（6）：1301-1304.
[14] 吴建龙，张艳利，乔建勇，等.高血压脑出血血肿周围脑组织中TNF-α、VEGF以及ET-1的表达及其意义[J].中华神经外科杂志，2017，33（5）：498-501.
[15] 王拥军.氧化应激与缺血性脑血管病[J].中国卒中杂志，2008，3（3）：163-165.
[16] 刘涛，宋敏，巩彦龙，等.中药对脑微血管内皮细胞影响的研究进展[J].时珍国医国药，2016（6）：1461-1463.
[17] 白蓉，王淑.注射用丹参多酚酸对脑缺血大鼠VEGF、IL-10的影响[J].中风与神经疾病，2016，33（5）：411-416.
[18] 徐芳琴，郭超，卞涛，等.丹红注射液对大鼠脑缺血/再灌注损伤治疗时间窗的影响[J].中成药，2016，38（9）：1887-1892.
[19] 刘家军，李小燕，杜成芬，等.丹参酮ⅡA磺酸钠对急性心肌梗死大鼠心肌组织间液钙离子与心功能的影响[J].安徽医药，2016，20（4）：643-646.
[20] 黑明燕，刘芙蓉，罗娅丽，等.丹参酮ⅡA对新生大鼠缺氧缺血性脑损伤磷酸化NMDA受体1表达及细胞内游离钙浓度的影响[J].中南大学学报：医学版，2010， 35（9）：940-946. |
|
|
|
