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| Diagnostic value of p504s, ck5/6 and p63 expression level in benign prostatic hyperplasia and prostate cancer |
| ZHANG Ying1 WANG Xizhe1 ZHOU Lu1 DING Fen′gan2▲ |
1.Department of Pathology, Huishan People's Hospital of Wuxi City, Jiangsu Province, Wuxi 214011, China;
2.Department of Pathology, Zhongda Hospital Affiliated to Dongnan University, Jiangsu Province, Nanjing 210009, China |
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Abstract Objective To study the diagnostic value of alpha methylacyl coenzyme A racemase (p504s), cytokeratin5/6 (ck5/6) and p63 in benign prostatic hyperplasia (BPH) and prostate cancer. Methods Immunohistochemical staining method was used to detect the expression of p504s, ck5/6 and p63 in 120 cases of prostate biopsy specimens that collected in Department of Pathology, Huishan People's Hospital of Wuxi City and Xishan People′s Hospital of Wuxi City from January 2012 to December 2017. In the 120 cases, 70 cases were prostate cancer (prostate cancer group), 50 cases were BPH (BPH group). 65 cases of patients with prostate acinic cell carcinoma were divided into three subgroups according to the Gleason classification standard: ≤6 scores group (20 cases), 7-8 scores group (26 cases) and ≥9 scores group (19 cases). The product of staining intensity and percentage of positive cells was compared among these groups. Statistical software SPSS 15.0 was used to analyze the results. Results The expression rate of p504s in the prostate cancer group was 91.43%, 64.28% (45/70) were strongly positive. Only 5 cases of BPH group were p504s weakly positive, and the expression rate was 10.00%. The positive rate of ck5/6 expression in prostate cancer group was 11.43% (8/70), and all of them were weakly positive. 90.00% (45/50) of BPH group were ck5/6 positive expression, and 84.00% (42/50) were positive expression above medium intensity. The positive rate of p63 expression in prostate cancer group was 8.57% (6/70), and all of them were weakly positive. 92.00% (46/50) of BPH group were p63 positive, and 82.00% (41/50) were positive expression above medium intensity. There was a significant difference in the expression of p504s, ck5/6 and p63 in the two groups (P ＜ 0.05). There was a significant difference in the expression intensity of p504s in ≤6 score group, 7-8 score group and ≥9 score group (P ＜ 0.05). The expression intensity of p504s in 7-8 score group and ≥9 score group were significantly higher than that in ≤6 score group (P ＜ 0.05). The expression intensity of p504s in 7-8 score group and ≥9 score group had no significant difference (P ＞ 0.05). There was no significant difference in the expression intensity of ck5/6 and p63 among three types of benign prostatic hyperplasia tissues, which included partial atrophy of glands, complete atrophy of glands, and hyperplasia of basal cells (P ＞ 0.05). There were 3 cases of ck5/6 deletion, and 2 cases of p63 expression deletion in the glandular partial atrophy. There were 2 cases of ck5/6 expression deletion, and 2 cases of p63 expression deletion in the complete atrophy of glands. Conclusion The expression of p504s is highly expressed in the prostate cancer tissue, while the expression of p63 and ck5/6 is decreased significantly. The expression intensity of p504s is enhanced with the degree increase of Gleason classification. It is suggested that the combined detection of p504s, ck5/6 and p63 has good application value in the diagnosis of prostate cancer and BPH.
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