基于生物信息学分析SLC19A3基因在乳腺癌中的表达意义及蟾毒灵的干预研究

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Abstract Objective To analyze the expression and its clinical significance of SLC19A3 gene in breast cancer and to explore the effects of Bufalin on the expression of SLC19A3 in breast cancer cells. Methods The datasets about breast cancer and effects of Bufalin on breast cancer cell were retrieved from Oncomine and GEO database, and the datasets were deeply mined and analyzed with literature. SLC19A3 mRNA expression between normal group and breast cancer group as well as correlation with prognosis were analyzed. Meanwhile, the effect of Bufalin on the expression level of SLC19A3 gene in breast cancer cells was also analyzed. Results The expression of SLC19A3 gene in breast cancer tissues was significantly higher than that of breast normal tissues through mining and analyzing the 9 datasets about SLC19A3 gene in breast carcinoma from Oncomine database (P ＜ 0.05). Survival analysis showed that the disease-free survival of breast cancer patients with higher expression of SLC19A3 gene was significantly better than the patients with lower expression of SLC19A3, and the prognosis of patients with high expression of SLC19A3 gene was better (P ＜ 0.05). Finally, the mRNA expression of SLC19A3 gene was up-regulated by Bufalin in the datasets of GSE85871 through mining the GEO database (P ＜ 0.01). Conclusion SLC19A3 gene is under-expressed in breast cancer and its expression level is related to the prognosis of breast cancer, which may be a new target of effects of Bufalin on breast cancer cells.

Key words： Bufalin Breast cancer SLC19A3 gene Data mining

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	LI Dan1 YU Tao2
	3 LI Jing1 ZHANG Fan1 ZENG Junfen1 PENG Yan1

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LI Dan1 YU Tao2,3 LI Jing1 ZHANG Fan1 ZENG Junfen1 PENG Yan1. Expression and clinical significance of SLC19A3 in breast cancer and effects of Bufalin on the expression of SLC19A3: analysis based on the data-mining from bioinformatics[J]. 中国医药导报, 2018, 15(17): 12-16.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2018/V15/I17/12

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